Construct: ORF TRCN0000466322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012753.1_s317c1
- Derived from:
- ccsbBroadEn_03704
- DNA Barcode:
- CGCACACTCACTTAATCGTGGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGA5 (56110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466322
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56110 | PCDHGA5 | protocadherin gamma subfami... | NM_032054.1 | 100% | 100% | |
| 2 | human | 56110 | PCDHGA5 | protocadherin gamma subfami... | NM_018918.2 | 86.7% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2508
- ORF length:
- 2439
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgagtcca cctaggggct ggggctgcgg agagctgctg ctgcccttca 121 tgctcctggg gacgctgtgc gagccaggat ccgggcagat ccgctactcg atgccggagg 181 agctggacaa aggctccttc gtcggcaaca tagccaagga ccttgggctg gagccccagg 241 agctggcgga gcgcggagtc cgcatcgtct ccagaggtag gacgcagctt tttgccctga 301 acccgcgaag cggcagcttg gtcaccgcgg gcaggataga ccgggaggag ctctgcgctc 361 agagcccact gtgtgtggtg aactttaaca tcttggttga gaacaaaatg aaaatttatg 421 gagtagaagt agaaataatc gatattaatg ataacttccc gcgtttccgg gatgaagagt 481 taaaagtaaa agttaatgaa aatgcggctg cagggacacg gttagtgctt cccttcgcgc 541 gggatgcgga tgtgggtgtg aactctctcc ggagttacca gctcagctcc aatctgcact 601 tctctctgga tgtggtaagc ggaactgatg gacaaaagta tccggagctg gtgttggaac 661 agcccctaga ccgcgagaaa gagactgttc acgacctcct cctcacagct ttagatggcg 721 gagacccggt actctccggc accacgcaca tccgtgttac ggtcctcgac gcaaacgaca 781 atgcgcccct gttcacccca tccgagtaca gcgtgagtgt tccagagaac atacctgtgg 841 gcactcggct gctcatgcta accgccacgg atccagatga gggaataaac gggaaattga 901 cctactcttt tcgcaatgaa gaagaaaaaa tttcggagac tttccaactt gattccaacc 961 tgggggaaat ctcaactcta caatcactgg actatgaaga atccagattc tacctcatgg 1021 aagtggtagc tcaggatgga ggcgctcttg ttgccagcgc taaggtggtg gtcacagtac 1081 aggacgtgaa tgacaatgcc cccgaagtga tcctcacctc tctgaccagt tcgatctctg 1141 aagactgtct tcccggaact gtaatcgcgc tgtttagcgt acatgatggt gattctggag 1201 aaaatggtga gattgcatgc tctattccta ggaatttgcc ttttaaattg gagaagtcag 1261 ttgataatta ctatcaccta ttaacaacta gggacctgga cagagaagag acttcagatt 1321 ataatatcac tttaaccgtc atggaccatg gaaccccgcc cctctctaca gaaagccaca 1381 tccccttgaa agtagcagac gttaatgaca acccacccaa tttccctcaa gcctcctact 1441 ccacctctgt cacagaaaac aatcccagag gtgtctctat cttctctgtg acagcccatg 1501 accccgacag cggcgacaac gctcgagtca cctactccct ggctgaagac acatttcagg 1561 gggcgccctt gtcctcctat gtatccatta actctgacac cggtgtcctg tatgctctga 1621 gatccttcga ctatgagcag ttgagagacc tacagttgtg ggtgacagcc agcgacagtg 1681 ggaaccctcc acttagcagc aacgtgtcgc tgagcctgtt tgtgctggac cagaacgaca 1741 atacgcctga gatcctgtac cccgccctcc ccacagacgg ttccacgggc gtggagctgg 1801 cgcctcgctc cgcagaacct ggctacctgg tgaccaaggt ggtagcggtg gacaaagatt 1861 caggccagaa cgcctggctg tcctaccgcc tgcttaaggc cagcgagcca ggactctttg 1921 cggttgggct gcacacgggc gaggtgcgca cagcgcgagc cctgctggac agagacgcgc 1981 tcaagcagag cctcgtggtg gccgtcgaag accatggcca gccccctctg tcagccacct 2041 tcacggtcac cgttgccgtg gccgacagga tccctgacat cctggctgac ctaggcagta 2101 tcaagacccc cattgaccct gaggatctgg acctcacact ctatcttgtg gtggcagtgg 2161 ctgcagtctc ctgcgtcttc ctggccttcg tcatcgtgct gctggtgcTC AGACTGAGGC 2221 GCTGGCACAA GTCACGCCTG CTTCAGGCTG AAGGCAGCAG GTTGGCGGGT GTGCCCGCCT 2281 CGCACTTTGT GGGCGTGGAT GGGGTTCGGG CTTTCCTGCA GACCTATTCC CACGAGGTCT 2341 CCCTCACCGC GGACTCGAGG AAGAGTCACC TGATCTTTCC CCAGCCCAAC TACGCAGACA 2401 CGCTCCTTAG TGAAGAGAGC TGTGAGAAAA GCGAGCCTCT TCTGATGTCT GATAAGGTAG 2461 ATGCAAACAA AGAAGAACGG CGAGTTCAGG TTAGTTTTCT CTTTCGGTTG CCAACTTTCT 2521 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2581 CGTAGTAATG AACTAGCCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2641 GTGGAAAGGA CGACGCACAC TCACTTAATC GTGGATCACG CGTTAAGTCg acaatcaacc 2701 tctggattac aaaatttgtg aaagatt