Construct: ORF TRCN0000466361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005043.1_s317c1
- Derived from:
- ccsbBroadEn_08440
- DNA Barcode:
- CATATCCCTGTTCATGTATCTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PINX1 (54984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54984 | PINX1 | PIN2 (TERF1) interacting te... | NM_017884.6 | 99.7% | 99.3% | 150A>C;644G>T |
| 2 | human | 54984 | PINX1 | PIN2 (TERF1) interacting te... | NM_001284356.1 | 52.9% | 42.4% | 150A>C;392_393ins77;522_523ins385 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1050
- ORF length:
- 984
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tatgctggct gaacgtcggc ggaagcagaa gtgggctgtg gatcctcaga 121 acactgcctg gagtaatgac gattccaagt ttggccagcg gatgctagag aagatggggt 181 ggtctaaagg aaagggttta ggggctcagg agcacggagc cacagatcat attaaagttc 241 aagtgaaaaa taaccacctg ggactcggag ctaccatcaa taatgaagac aactggattg 301 cccatcagga tgattttaac cagcttctgg ccgaactgaa cacttgccat gggcaggaaa 361 ccacagattc ctcggacaag aaggaaaaga aatcttttag ccttgaggaa aagtccaaaa 421 tctccaaaaa ccgtgttcac tatatgaaat tcacaaaagg gaaggatctg tcatctcgga 481 gcaaaacaga tcttgactgc atttttggga aaagacagag taagaagact cccgagggcg 541 atgccagtcc ctccactcca gaggagaacg aaaccacgac aaccagcgcc ttcaccatcc 601 aggagtactt tgccaagcgg atggcagcac tgaagaacaa gccccaggtt ccagttccag 661 ggtctgacat ttctgagacg caggtggaac gtaaaagggg gaagaaaata aataaagagg 721 ccacaggtaa agatgtggaa agttacctcc agccTAAGGC CAAGAGGCAC ACGGAGGGAA 781 AGCCCGAGAG GGCCGAGGCC CAGGAGCGAG TGGCCAAGAA GAAGAGCGCG CCAGCAGAAG 841 AGCAGCTCAG AGGCCCCTGC TGGGACCAGA GTTCCAAGGC CTCTGCTCAG GATGCAGGGG 901 ACCATGTGCA GCCGCCTGAG GGCCGGGACT TCACCCTGAA GCCCAAAAAG AGGAGAGGGA 961 AGAAAAAGCT GCAAAAACCA GTAGAGATAG CAGAGGACGC TACACTAGAA GAAACGCTAG 1021 TGAAAAAGAA GAAGAAGAAA GATTCCAAAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1081 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1141 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACATAT 1201 CCCTGTTCAT GTATCTACTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1261 tgaaagatt