Construct: ORF TRCN0000466426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008776.1_s317c1
- Derived from:
- ccsbBroadEn_15865
- DNA Barcode:
- TGGAGAAAAACCACCTATAAAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ING3 (54556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466426
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54556 | ING3 | inhibitor of growth family ... | NM_198267.2 | 100% | 100% | |
| 2 | human | 54556 | ING3 | inhibitor of growth family ... | XM_017012369.2 | 98.9% | 98.9% | 266_267insGCA |
| 3 | human | 54556 | ING3 | inhibitor of growth family ... | NM_019071.3 | 21.7% | 21.5% | 268_273delGTAGAT;275_276delGAinsAG;282_1254delinsC |
| 4 | human | 54556 | ING3 | inhibitor of growth family ... | XR_001744819.1 | 13% | (many diffs) | |
| 5 | mouse | 71777 | Ing3 | inhibitor of growth family,... | NM_001311061.1 | 20.7% | 21.9% | (many diffs) |
| 6 | mouse | 71777 | Ing3 | inhibitor of growth family,... | NM_023626.4 | 20.1% | 21.3% | (many diffs) |
| 7 | mouse | 71777 | Ing3 | inhibitor of growth family,... | XM_006505166.3 | 14.8% | 15.6% | (many diffs) |
| 8 | mouse | 71777 | Ing3 | inhibitor of growth family,... | XM_017321747.1 | 12.4% | 13% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 105
- ORF end:
- 381
- ORF length:
- 276
- Sequence:
-
1 GTTCATTCTC AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG 61 AGGCTAGCAT CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCATGTTG TACCTAGAAG 121 ACTATCTGGA AATGATTGAG CAGCTTCCTA TGGATCTGCG GGACCGCTTC ACGGAAATGC 181 GCGAGATGGA CCTGCAGGTG CAGAATGCAA TGGATCAACT AGAACAAAGA GTCAGTGAAT 241 TCTTTATGAA TGCAAAGAAA AATAAACCTG AGTGGAGGGA AGAGCAAATG GCATCCATCA 301 AAAAAGACTA CTATAAAGCT TTGGAAGATG CAGATGAGAA GGTTCAGTTG GCAAACCAGA 361 TATATGACTT GCAGCACTTC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGGA GAAAAACCAC 541 CTATAAAAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt