Construct: ORF TRCN0000466433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000273.1_s317c1
- Derived from:
- ccsbBroadEn_09323
- DNA Barcode:
- GCAACTTACGGCCTCTTTCCACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARVELD3 (91862)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91862 | MARVELD3 | MARVEL domain containing 3 | NM_052858.6 | 99.8% | 99.7% | 178G>A;1134T>G |
| 2 | human | 91862 | MARVELD3 | MARVEL domain containing 3 | XM_011523449.3 | 53.7% | 49.6% | (many diffs) |
| 3 | human | 91862 | MARVELD3 | MARVEL domain containing 3 | NM_001271329.2 | 36.4% | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1269
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agatccgtcg ggggctcgcg agccccgggc ccggccgaga gagcgggacc 121 cgggacggcg cccccaccca gaccaaggcc gcacccacga tcgaccgcgg gaccgacccg 181 gggacccgcg caggaagcga agcagcgacg ggaaccggcg aagggacggg gaccgggacc 241 cgaagagaga ccaggagagg gacgggaacc gcgaccggaa ccgggaccgg gagagggaga 301 gagagaggga aagagacccg gaccgaggcc cccgccggga cacacacagg gacgcgggcc 361 ctcgcgcagg tgaacacgga gtttgggaaa aaccgcgcca aagccggacg cgggacggag 421 cccggggact gacctgggac gcagccgcgc ctcctgggcc cgcgccctgg gaagccccgg 481 agccgccgca gccgcagagg aagggagacc ccgggcgccg cagacccgaa agtgaacccc 541 cttcggagag atatctgccc tcgaccccca ggcctggacg agaggaggtg gaatattacc 601 agtcagaggc ggaaggactc ctggaatgcc acaaatgcaa atacttgtgc actgggagag 661 cctgctgcca aatgctggag gttctcctga acttgctgat cctggcctgc agctctgtgt 721 cttacagttc cacagggggc tacacgggca tcaccagctt ggggggcatt tactactatc 781 agttcggagg ggcttacagt ggctttgatg gtgctgacgg ggagaaggcc cagcaactgg 841 atgtccagtt ctaccagcta aagctgccca tggtcactgt ggcaatggcc tgtagtggag 901 ccctcacagc ccTCTGCTGC CTCTTCGTTG CCATGGGTGT CCTGCGGGTC CCGTGGCATT 961 GTCCACTGTT GCTGGTGACC GAAGGCTTGT TGGACATGCT CATCGCGGGG GGGTACATCC 1021 CGGCCTTGTA CTTCTACTTC CACTACCTCT CTGCTGCCTA TGGCTCTCCT GTGTGTAAAG 1081 AGAGGCAGGC GCTGTACCAA AGCAAAGGCT ACAGCGGTTT CGGCTGCAGT TTCCACGGAG 1141 CAGATATAGG AGCTGGAATC TTTGCTGCCC TGGGCATTGT GGTCTTTGCC CTGGGGGCGG 1201 TCCTGGCCAT AAAGGGCTAC CGAAAAGTTA GGAAGCTAAA AGAGAAGCCA GCAGAAATGT 1261 TTGAATTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCAACT TACGGCCTCT TTCCACTCAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt