Construct: ORF TRCN0000466479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002434.1_s317c1
- Derived from:
- ccsbBroadEn_12112
- DNA Barcode:
- TATGCTCGCCCTCGCGTCCTGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN32 (54925)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | NM_001324346.2 | 37.4% | 28.5% | (many diffs) |
| 2 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | NM_001284527.2 | 36.7% | 36.2% | 1_3delATG;758_759delGT;774_2091del |
| 3 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | XM_011522555.2 | 36.7% | 36.2% | 1_3delATG;758_759delGT;774_2091del |
| 4 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | XM_017023371.2 | 36.7% | 36.2% | 1_3delATG;758_759delGT;774_2091del |
| 5 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | XM_005255403.2 | 24.2% | 23.8% | (many diffs) |
| 6 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | XM_017023372.1 | 24.2% | 23.8% | (many diffs) |
| 7 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | XM_017023373.2 | 24.2% | 23.8% | (many diffs) |
| 8 | human | 54925 | ZSCAN32 | zinc finger and SCAN domain... | NM_001324343.2 | 17.8% | 17.3% | 0_1ins396;359_360delGT;375_1692del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagtgaag agtaccgagg cacacccatc ctcaaacaag gatcccacac 121 agggccagaa atcagccctc cagggtaaca gccctgactc cgaggcctcc cgtcagcgct 181 tcaggcagtt ttgctaccag gaggtaactg gcccacatga agcttttagc aaactctggg 241 aactctgttg tcagtggctg aggccgaaga cccactcaaa agaggaaatc ctggagctgc 301 tggttttgga gcagtttctg actatcttgc cagaggagat ccagacctgg gtgagggagc 361 agcatccaga aaacggcgag gaagctgtgg ctctgGTTGA GGATGTACAG AGAGCTCCTG 421 GACAACAGGT TCTAGATTCT GAGAAGGACT TGAAAGTACT CATGAAGGAG ATGGCCCCTT 481 TGGGAGCAAC CAGAGAATCA CTGAGATCCC AATGGAAACA GGAGGTTCAG CCAGAGGAAC 541 CGACTTTTAA GGGATCACAG AGCTCACACC AAAGACCAGG GGAACAGTCA GAAGCCTGGC 601 TTGCTCCTCA GGCTCCCAGG AACCTGCCTC AAAACACAGG TCTCCACGAC CAGGAGACAG 661 GTGCTGTGGT CTGGACAGCT GGGTCCCAGG GACCAGCCAT GCGTGACAAC AGAGCTGTAT 721 CCCTCTGTCA GCAAGAATGG ATGTGCCCAG GCCCTGCACA AAGGGCCCTC TACAGGGGTG 781 CCACCCAGAG GAAGGACAGT CACGTCTCGC TGGCAACAGG TGCCCTGGGG CTATGCCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAT ATGCTCGCCC TCGCGTCCTG GCAACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att