Construct: ORF TRCN0000466515
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007326.1_s317c1
- Derived from:
- ccsbBroadEn_08311
- DNA Barcode:
- TTAGCCGATTTTAAACGTCAGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTFP1 (51537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466515
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51537 | MTFP1 | mitochondrial fission proce... | NM_016498.5 | 99.7% | 100% | 234T>C |
| 2 | human | 51537 | MTFP1 | mitochondrial fission proce... | NM_001003704.3 | 41.5% | 42.3% | 193_194ins233;266_405del |
| 3 | mouse | 67900 | Mtfp1 | mitochondrial fission proce... | NM_026443.4 | 86.9% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agagccgcag ccgcggggcg cagagcgcga tctctaccgg gacacgtggg 121 tgcgatacct gggctatgcc aatgaggtgg gcgaggcttt ccgctcTCTT GTGCCAGCGG 181 CGGTGGTGTG GCTGAGCTAT GGCGTGGCCA GCTCCTACGT GCTGGCGGAT GCCATTGACA 241 AAGGCAAGAA GGCTGGAGAG GTGCCCAGCC CTGAAGCAGG CCGCAGCGCC AGGGTGACCG 301 TGGCTGTGGT GGACACCTTT GTATGGCAGG CTCTAGCCTC TGTGGCCATT CCGGGCTTCA 361 CCATCAACCG CGTGTGTGCT GCCTCTCTCT ATGTCCTGGG CACTGCCACC CGCTGGCCCC 421 TGGCTGTCCG CAAGTGGACC ACCACCGCGC TTGGGCTGTT GACCATCCCC ATCATTATCC 481 ACCCCATTGA CAGGTCGGTG GATTTCCTCC TGGACTCCAG CCTGCGCAAG CTCTACCCAA 541 CAGTGGGGAA GCCCAGCTCC TCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TAGCCGATTT 721 TAAACGTCAG TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att