Construct: ORF TRCN0000466544
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016757.1_s317c1
- Derived from:
- ccsbBroadEn_11534
- DNA Barcode:
- GCTTTGTCCAGGTGACGATCGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGF2BP3 (10643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466544
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10643 | IGF2BP3 | insulin like growth factor ... | NM_006547.3 | 16.8% | 16.4% | (many diffs) |
| 2 | human | 10643 | IGF2BP3 | insulin like growth factor ... | XM_011515089.2 | 16.8% | 16.3% | (many diffs) |
| 3 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | NM_023670.3 | 15.3% | 14.8% | (many diffs) |
| 4 | mouse | 140488 | Igf2bp3 | insulin-like growth factor ... | XR_377421.3 | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 390
- ORF length:
- 300
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAACAAACT GTATATCGGA AACCTCAGCG 121 AGAACGCCGC CCCCTCGGAC CTAGAAAGTA TCTTCAAGGA CGCCAAGATC CCGGTGTCGG 181 GACCCTTCCT GGTGAAGACT GGCTACGCGT TCGTGGACTG CCCGGACGAG AGCTGGGCCC 241 TCAAGGCCAT CGAGGCGCTT TCAGGTAAAA TAGAACTGCA CGGGAAACCC ATAGAAGTTG 301 AGCACTCGGT CCCAAAAAGG CAAAGGATTC GGAAACTTCA GATACGAAAT ATCCCGCCTC 361 ATTTACAGTG GGAGGAAATG GGGCAGCCTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCTTT 541 GTCCAGGTGA CGATCGGTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt