Construct: ORF TRCN0000466555
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008402.1_s317c1
- Derived from:
- ccsbBroadEn_13618
- DNA Barcode:
- GCCTTGCACGCTAATAGATGGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKDD1A (348094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466555
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 348094 | ANKDD1A | ankyrin repeat and death do... | NM_182703.6 | 76.3% | 76.4% | 1_273del;1063_1158del;1548T>C |
| 2 | human | 7576 | ZNF28 | zinc finger protein 28 | NM_001369767.1 | 6.5% | 4.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1266
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatgcgctt ctcctgtctg cctggttcgg ccacttacga atcctccaga 121 tcttggtaaa ctcaggggcc aagatccact gtgagagcaa ggatggcctg accttactgc 181 actgcgcagc ccaaaaaggc catgtgcctg tgctggcgtt cataatggag gacctggagg 241 atgtggccct ggaccacgta gacaagctgg ggaggacggc gtttcacagg gcagctgagc 301 acgggcagct ggatgctctg gacttcctcg tgggctctgg ctgtgaccac aatgtcaaag 361 acaaggaggg gaacactgcc cttcatctgg ctgctggtcg gggccatatg gctgtgctgc 421 agcgacttgt ggacatcggg ctggacctgg aggagcagaa tgcggaaggt ctgactgccc 481 tgcattcggc tgctggagga tcccaccctg actgtgtgca gctcctcctc agggctggga 541 gcaccgtgaa tgccctcacc cagaaaaacc taagctgcct tcactatgca gccctcagtg 601 gctcggagga tgtgtctcgg gtcctcatcc acgcaggagg ctgcgccaac gtggttgatc 661 atcagggtgc ctctcctctg cacctcgctg tgaggcacaa cttccctgcc ttggtccggc 721 tcctcatcaa ctccgacagt gacgtgaatg ccgtggacaa taggcagcag acgccccttc 781 acctggctgc agagcacgcc tggcaggaca tagcagatat gctcctcatt gctggggttg 841 acttaaacct gagagataag gaccacccca gtgatccctc tgggaagagc ttgtccttta 901 agcaggacca tcggcaggaa acacagcagc tccgttctgt gctgtggcgg ctggcctCCA 961 GGTATCTGCA GCCCCGTGAG TGGAAGAAGC TGGCATATTC CTGGGAGTTC ACGGAGGCAC 1021 ATGTCGACGC CATCGAGCAA CAGTGGACAG GCACCAGGAG CTATCAGGAG CACGGCCACC 1081 GAATGCTGCT CATTTGGCTG CATGGCGTGG CCACGGCTGG TGAGAACCCC AGCAAAGCGC 1141 TGTTCGAGGG CCTCGTGGCC ATTGGCAGGA GGGACCTGGC TGGCTGGAGT ACAATGGCGA 1201 GATCTCAGCT CACGGCAACC TCCGCCTCCC GGGTTCAAAT GATTCTCGTG CCTCAGCCTC 1261 CCGAGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCTTGCAC GCTAATAGAT GGTATACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt