Construct: ORF TRCN0000466584
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000707.1_s317c1
- Derived from:
- ccsbBroadEn_04985
- DNA Barcode:
- TGGGTAAAAGCGGACTAATTTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEKT5 (146279)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466584
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146279 | TEKT5 | tektin 5 | NM_144674.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1524
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtttctt gggactactc agaccgccag ttactgtggt cccaagaaat 121 gctgtggctt gacctcactg ccagctgtac aggcgccagt gatccaggaa tgctatcagc 181 cctactacct gcccgggtac cgctacctca attcatggag gcctagcctc ttctacaaga 241 tagccaacgt ccagacctgc ccggacgaga gcaccagtac cctgcggccg cccaccatcc 301 tgcccacact gcgctccgca ctcttctctc gctatagccc ccacgactgg gaccagtcca 361 accagctgca ggtgcgtggg gccgaggcct cccggctgtg ggccagccgg ctgacggatg 421 actccatgag gctcttgcag gacaaggacc agctgacgca ccagatgcag gagggcacct 481 gccggaacct gggccagagg ctgtcggaca ttggcttctg gaagtcagag ctgagctatg 541 agctggacag gcttctgact gagaaccaga acttggagac ggtcaagagg cggctggagt 601 gcgcggccaa tgaggtgaac tgcccattgc aggtggcctt ggagtgtctg taccatcgag 661 agaagaggat tgggattgat ttggtccatg acaacgtgga gaaaaacctt atccgggaag 721 tggatttgct aaaatgttgc caagaacaga tgagaaaatt agctcaaaga attgatatcc 781 agatgcggga taaccgggat gctcagcacg tgctggagag ggacctcgaa gacaaaagct 841 cggcccagtg tatcgatgag aagtgcttta acctgagaaa tacgtcagac tgcatcagct 901 tcttccacgg catggagaaa attgacggca cgatctccgt acctgagacc tgggccaagt 961 tcagtaacga caacatcaaa cactctcaga acatgcgggc caactccatc cagctgcggg 1021 aggaggcgga gcacctcttt gagaccttgt cggatcagat gtggaggcag ttcacagaca 1081 ccaacctggc cttcaacgcc cgcatctctg aggtgacgga tgtgaagaat aagctgcaga 1141 cgcagctggc gaagacgctg caggagaTCT TCCAGGCCGA GAACACCATC ATGCTGCTGG 1201 AAAGGTCCAT CATGGCCAAG GAGGGCCCGC TGAAGGTGGC CCAGACAAGG CTGGAGTGCC 1261 GGACCCGGCG CCCCAACATG GAGCTGTGCA GGGACATCCC GCAGTTGAAG CTGGTGAACG 1321 AGGTGTTCAC CATCGACGAC ACCCTGCAGA CCCTCAAGCT GCGGCTGCGG GAGACACAGG 1381 ACACGCTGCA GCTGCTGGTC ATGACCAAGT GCCGGCTGGA GCACGAGCTC GCCATCAAGG 1441 CCAACACCCT CTGCATCGAC AAGGAGAAGT GCATGGGCAT GCGTAAGACC TTCCCCTGCA 1501 CCCCGCGCCT GGTGGGCCAC ACCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1561 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1621 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GGGTAAAAGC 1681 GGACTAATTT CGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1741 att