Construct: ORF TRCN0000466598
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006680.1_s317c1
- Derived from:
- ccsbBroadEn_10984
- DNA Barcode:
- CTGGGAGCCTCGTGGGTCATTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYO10 (4651)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466598
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4651 | MYO10 | myosin X | XM_006714475.3 | 4.6% | 4.6% | (many diffs) |
| 2 | human | 4651 | MYO10 | myosin X | NM_012334.3 | 4.5% | 4.6% | (many diffs) |
| 3 | mouse | 17909 | Myo10 | myosin X | NM_019472.2 | 4% | 4.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 357
- ORF length:
- 291
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA TAACTTCTTC ACCGAGGGAA CACGGGTCTG GCTGAGAGAA AATGGCCAGC 121 ATTTTCCAAG TACTGTAAAT TCCTGTGCAG AAGGCATCGT CGTCTTCCGG ACAGACTATG 181 GTCAGGTATT CACTTACAAG CAGAGCACAA TTACCCACCA GAAGGTGACT GCTATGCACC 241 CCACGAACGA GGAGGGCGTG GATGACATGG CGTCCTTGAC AGAGCTCCAT GGCGGCTCCA 301 TCATGTATAA CTTATTCCAG CGGTATAAGA GAAATCAAAT ATATGTGCAG ATAGGTTGCC 361 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 421 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 481 ATATATCTTG TGGAAAGGAC GACTGGGAGC CTCGTGGGTC ATTAACACGC GTTAAGTCga 541 caatcaacct ctggattaca aaatttgtga aagatt