Construct: ORF TRCN0000466620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001224.1_s317c1
- Derived from:
- ccsbBroadEn_06872
- DNA Barcode:
- AGTATCGATACGGTGATAAGACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROM1 (6094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466620
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6094 | ROM1 | retinal outer segment membr... | NM_000327.3 | 99.8% | 100% | 669G>C;676C>T |
| 2 | mouse | 19881 | Rom1 | rod outer segment membrane ... | NM_009073.4 | 85.1% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccggtgttg cccctggtgc tgcccctgca gccccgcatc cgcctggcac 121 aagggctctg gctcctctcc tggctgctgg cgctggctgg tggcgtcatc ctcctctgta 181 gtgggcacct cctggtccag ctaaggcacc ttggcacctt cctggctccc tcctgtcagt 241 tccctgtcct gccccaggct gccctggcag cgggcgcggt ggctctgggc acaggactag 301 tgggtgtagg agccagccgg gcaagtctga atgcagctct ataccctccc tggcgagggg 361 tcctgggccc gctgctggtg gctggcacgg ctggtggggg ggggctcctg gtcgtcgccc 421 tcgggctagc cctggctttg cctgggagtc tggatgaggc gctggaggag ggcctggtga 481 ctgccttggc tcactacaag gacacagagg tgcctgggca ctgtcaggcc aaaaggctgg 541 tggatgagct gcaactgagg taccactgct gcgggcgcca cgggtacaag gattggtttg 601 gggtccagtg ggtcagcagc cgttacctgg atcccggtga ccgggatgtg gctgaccgga 661 tccagagcaa tgtagaaggc ctatacctga ctgatgggGT CCCTTTCTCC TGTTGCAACC 721 CCCACTCACC CCGCCCTTGC TTGCAAAACC GTCTTTCAGA CTCCTACGCC CACCCCCTGT 781 TCGATCCCCG ACAACCCAAC CAAAACCTCT GGGCCCAAGG GTGCCATGAG GTGCTGCTGG 841 AGCACTTGCA GGACTTGGCA GGCACACTGG GTAGCATGCT GGCTGTCACC TTCCTACTGC 901 AGGCTCTGGT GCTCCTTGGC CTGCGGTACC TGCAAACAGC ACTGGAGGGG CTTGGAGGGG 961 TCATTGATGC GGGAGGAGAG ACCCAGGGCT ATCTCTTTCC CAGTGGGCTG AAAGATATGC 1021 TGAAAACAGC ATGGCTACAG GGAGGGGTTG CCTGCAGGCC AGCACCTGAG GAGGCCCCAC 1081 CAGGAGAAGC ACCTCCCAAG GAGGATCTAT CTGAGGCCTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAAGTATC GATACGGTGA TAAGACTGAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt