Construct: ORF TRCN0000466624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004916.1_s317c1
- Derived from:
- ccsbBroadEn_03143
- DNA Barcode:
- TTGAGTTTAACAAAGCCTCTCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMARCAL1 (50485)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466624
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50485 | SMARCAL1 | SWI/SNF related, matrix ass... | NM_001127207.2 | 100% | 100% | |
| 2 | human | 50485 | SMARCAL1 | SWI/SNF related, matrix ass... | NM_014140.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2928
- ORF length:
- 2862
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cttgcctctt acagaggagc agaggaaaaa gattgaagag aatcgacaaa 121 aggctctggc ccgcagagct gagaagttat tggcagaaca gcatcagagg actagctcgg 181 gcacctccat tgctggcaac ccattccagg ccaagcaagg cccatcccaa aatttcccaa 241 gggagtcttg taagccagtg agccatggtg tcattttcaa gcaacagaat ctcagtagct 301 catctaatgc tgaccaaaga cctcatgatt cccacagttt tcaggcaaag ggaatatgga 361 aaaagccaga agaaatgccc acagcctgcc caggccacag tccacgtagt caaatggctc 421 tcactggaat ctctcctccc ttggcacaaa gtcctccaga ggtccctaaa caacagctct 481 tgagttatga gttaggtcaa ggtcatgctc aggcttcacc tgagatcagg ttcacaccct 541 ttgctaaccc aactcataag cctctggcca aaccaaagag ttcccaagag acaccagctc 601 attcctctgg acagcctccc agggatgcta agttagaggc caagacagca aaagcctccc 661 cttcggggca gaacatttct tacatccatt ctagctcaga gagtgtaacg cccaggacag 721 aaggaagact ccagcagaag tcagggtcct cagtccaaaa aggagtgaac tctcagaagg 781 gaaagtgcgt aaggaacggc gatcgtttcc aggtgttgat tgggtacaat gcggaactca 841 ttgcagtgtt taagaccctg cccagcaaga attatgatcc tgacaccaag acgtggaact 901 tcagcatgaa tgactatagt gccctgatga aagcagccca gagcctcccc acggtcaacc 961 tgcagcctct ggaatgggcc tatggcagca gcgagtcacc ctccaccagc agtgagggac 1021 aggccggcct tccatcagct ccatcccttt catttgtcaa agggcgatgc atgctcatct 1081 ccagggccta cttcgaggca gacatcagtt attcacagga ccttattgcg ctttttaaac 1141 agatggattc cagaagatat gatgtcaaga ccaggaagtg gagctttctc ttggaagagc 1201 acagtaaact aattgcaaag gtgcgctgcc tcccacaagt tcagctggac cctctgccca 1261 cgactctcac cctggcgttt gcttctcagc tcaagaagac atctctcagt ctcacgccag 1321 atgtcccaga ggcagacctt tctgaagtgg accccaagct cgtgtctaat ctgatgccct 1381 ttcagagagc tggagtcaat tttgccatag ccaaaggagg ccgcctgctg ctcgctgacg 1441 acatgggcct ggggaagacc atccaagcca tctgcatcgc agccttttac cggaaggagt 1501 ggccgctcct ggtggtggtg ccatcctccg tgcgcttcac ctgggagcag gccttccttc 1561 ggtggctgcc atctctgagc ccagattgca tcaacgtcgt ggtgactggg aaggaccgcc 1621 tgacagctgg cctgatcaac attgtcagct ttgaccttct tagcaagttg gaaaaacagc 1681 taaaaacccc ttttaaagtt gtcatcattg atgaatctca cttcctcaaa aacagtagga 1741 ctgcccgctg tcgagcagct atgccggtcc taaaggttgc caagagggtg atcctgttgt 1801 cgggcacacc agccatgtcc cggcccgcag agctctacac gcagatcatc gcagtcaagc 1861 caactttctt cccccagttt catgcctttg gacttcgcta ctgtgatgcc aaacggatgc 1921 cttgggggtg ggactactca ggttcctcca acctgggaga gctgaagctc ctgctggagg 1981 aagcagtcat gctgcggcgc ctcaagtccg acgtcctttc ccagctgcct gccaagcagc 2041 gcaagatagt ggtgattgcc ccaggacgga tcaatgccag gaccagagct gccctggatg 2101 ctgcagccaa ggaaatgacc accaaggaca aaactaaaca gcagcagaaa gatgccctca 2161 ttctcttctt caacagaaca gctgaagcta aaatcccatc tgtcattgaa tatatcttgg 2221 acctactgga aagtggaaga gagaagtttt tagtatttgc acaccataag gtggtcctgg 2281 acgcaattac gcaagagctt gagagaaagc acgtgcagca catccgcatc gatggctcca 2341 cctcatcagc tgagcgggag gacctgtgcc agcagttcca actgtcggag aggcatgctg 2401 tggccgtgct gtccatcacc gctgccaata tgggcctcac cttctcctcg gctgacctgg 2461 tggtgtttgc tgagctgttt tggaacccag gggtgctgat ccaggctgag gaccgcgtgc 2521 accgcattgg acagaccagc tccgtgggca ttcactacct cgtggcaaag ggcacagctg 2581 atgactacct ttggccccTG ATTCAAGAGA AGATTAAAGT TCTGGCAGAA GCCGGGCTTT 2641 CTGAGACCAA TTTTTCAGAA ATGACAGAAT CCACTGATTA CCTCTACAAG GACCCAAAGC 2701 AGCAGAAGAT CTACGACCTA TTCCAGAAGT CCTTTGAGAA AGAAGGAAGT GATATGGAGC 2761 TCCTGGAAGC AGCAGAGTCC TTTGACCCAG GAAGTGCTTC AGGAACATCT GGAAGTAGTT 2821 CCCAGAACAT GGGAGACACC CTGGATGAAA GCTCATTGAC AGCCAGTCCA CAGAAGAAAA 2881 GGAGATTTGA ATTTTTTGAT AACTGGGACA GCTTTACGTC TCCCCTGTAC CCAACTTTCT 2941 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3001 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3061 GTGGAAAGGA CGATTGAGTT TAACAAAGCC TCTCTTTACG CGTTAAGTCg acaatcaacc 3121 tctggattac aaaatttgtg aaagatt