Construct: ORF TRCN0000466630
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018429.1_s317c1
- Derived from:
- ccsbBroadEn_05697
- DNA Barcode:
- CCTTGAGCCACAAACATTCCCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHAC2 (494143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466630
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 494143 | CHAC2 | ChaC cation transport regul... | NM_001008708.4 | 100% | 100% | |
| 2 | human | 494143 | CHAC2 | ChaC cation transport regul... | NM_001346127.1 | 69.5% | 69% | 0_1ins163;2_3insCCTGC |
| 3 | mouse | 68044 | Chac2 | ChaC, cation transport regu... | NM_026527.3 | 88.7% | 90.7% | (many diffs) |
| 4 | mouse | 68044 | Chac2 | ChaC, cation transport regu... | NM_001290667.1 | 82.6% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggtttttggt tacgggtccc tgatctggaa ggtggatttc ccctatcagg 121 acaagctggt cggatacatc accaactaca gcaggcgctt ctggcagggc agcacggacc 181 accgcggggt ccccggcaag cctggaagag ttgtgactct tgttgaagat ccTGCGGGAT 241 GTGTATGGGG TGTTGCTTAC AGATTGCCAG TAGGAAAGGA AGAAGAAGTA AAAGCATACC 301 TTGACTTCAG AGAAAAAGGA GGCTACAGAA CCACAACAGT CATTTTTTAT CCAAAAGATC 361 CCACAACAAA ACCATTCAGT GTATTGCTAT ATATTGGAAC ATGTGATAAT CCTGATTATC 421 TTGGTCCTGC ACCTCTGGAA GACATTGCTG AACAAATTTT TAATGCAGCT GGTCCAAGTG 481 GAAGAAATAC AGAATATCTT TTTGAACTTG CAAATTCTAT TAGGAACCTT GTGCCAGAAG 541 AAGCAGATGA GCATCTTTTC GCTTTGGAAA AATTAGTAAA GGAACGTTTA GAAGGGAAAC 601 AGAACCTCAA TTGCATATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCTTGAG CCACAAACAT 781 TCCCACAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt