Construct: ORF TRCN0000466694
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015106.1_s317c1
- Derived from:
- ccsbBroadEn_13710
- DNA Barcode:
- ATTAATCGAAACTGTCCCCATAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BOLA2 (552900)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466694
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 654483 | BOLA2B | bolA family member 2B | NM_001039182.4 | 66.2% | 62.7% | (many diffs) |
| 2 | human | 654483 | BOLA2B | bolA family member 2B | NM_001320607.2 | 63.6% | 41.7% | (many diffs) |
| 3 | human | 552900 | BOLA2 | bolA family member 2 | NR_135304.1 | 41% | (many diffs) | |
| 4 | human | 552900 | BOLA2 | bolA family member 2 | NM_001031827.2 | 37.5% | 35.5% | (many diffs) |
| 5 | human | 552900 | BOLA2 | bolA family member 2 | NM_001320579.1 | 32.6% | 21% | (many diffs) |
| 6 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320623.1 | 20% | 18.7% | (many diffs) |
| 7 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320622.1 | 14.2% | 9.8% | 1_198del;260_261ins38;335_915del |
| 8 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NR_135316.1 | 9.6% | (many diffs) | |
| 9 | mouse | 66162 | Bola2 | bolA-like 2 (E. coli) | NM_175103.3 | 59% | 58.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 264
- ORF length:
- 174
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGAACTCAG CGCCGAATAC CTCCGCGAGA 121 AGCTGCAGCG GGACCTGGAG GCGGAGCATG TGGAGGTGGA GGACACGACC CTCAACCGTT 181 GCTCCTGTAG CTTCCGAGTC CTGGTGGTGT CGGCCAAGTT CGAGGGGAAA CCGCTGCTTC 241 AGAGACACAG GTTCTGTACA GAGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 301 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 361 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TTAATCGAAA 421 CTGTCCCCAT AGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 481 att