Construct: ORF TRCN0000466723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017084.1_s317c1
- Derived from:
- ccsbBroadEn_14251
- DNA Barcode:
- ACACGTATCCGACAAAGGCGTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL1 (65008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466723
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65008 | MRPL1 | mitochondrial ribosomal pro... | NM_020236.4 | 93.1% | 92.9% | 1_66del;530T>C |
| 2 | human | 65008 | MRPL1 | mitochondrial ribosomal pro... | XR_001741317.1 | 56.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ttatcagaca tcactttgtt cttgttctgt aaacatccga gtgcccaaca 121 gacattttgc tgctgctaca aagtctgcaa agaaaacaaa aaaaggtgct aaagaaaaaa 181 caccagatga gaaaaaagat gaaatagaaa aaataaaagc atatccctat atggaaggcg 241 aacctgagga tgatgtctat ttaaaacgct tatacccgag acagatatat gaggtggaga 301 aagctgttca cttacttaag aaatttcaaa ttcttgactt tactagtcca aagcaaagtg 361 tttatcttga tttgacactg gatatggcac tgggaaagaa gaaaaacgtg gagccattta 421 ccagtgttct tagtttgcca tacccatttg cttccgaaat caataaagtt gctgtattta 481 cagagaatgc atcagaggtc aaaatagcgg aagaaaatgg agctgcatct gcaggaggca 541 ctagtctgat acagaagatt tgggatgatg aaattgttgc agactttTAC GTAGCTGTTC 601 CAGAAATAAT GCCTGAACTT AATCGATTAA GGAAGAAACT GAATAAAAAA TATCCAAAGC 661 TTTCTCGAAA TTCCATTGGC CGTGACATCC CCAAAATGCT TGAATTATTT AAAAATGGAC 721 ATGAAATTAA GGTAGATGAA GAAAGGGAGA ACTTTCTCCA GACCAAAATA GCAACATTGG 781 ATATGTCAAG TGACCAGATA GCTGCCAATC TGCAAGCAGT TATTAATGAA GTTTGTAGGC 841 ACAGACCGCT GAATTTGGGT CCCTTTGTGG TACGTGCTTT CCTTCGTAGT TCAACAAGTG 901 AAGGTTTATT ACTGAAGATT GATCCATTGT TGCCTAAAGA AGTAAAAAAT GAAGAAAGTG 961 AAAAAGAAGA TGCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACACGTATCC GACAAAGGCG 1141 TAAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt