Construct: ORF TRCN0000466776

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008899.1_s317c1
Derived from:
ccsbBroadEn_06568
DNA Barcode:
ATTTATATGCAAAAGGCAACAAGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCM2 (4171)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466776
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4171 MCM2 minichromosome maintenance ... NM_004526.4 99.9% 100% 504C>T
2 human 4171 MCM2 minichromosome maintenance ... XM_024453531.1 98.9% 99% 0_1ins27;477C>T
3 human 4171 MCM2 minichromosome maintenance ... NR_073375.2 78.5% (many diffs)
4 mouse 17216 Mcm2 minichromosome maintenance ... NM_008564.2 87.5% 95.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2778
ORF length:
2712
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggaatcatcg gaatccttca ccatggcatc cagcccggcc cagcgtcggc
121gaggcaatga tcctctcacc tccagccctg gccgaagctc ccggcgtact gatgccctca
181cctccagccc tggccgtgac cttccaccat ttgaggatga gtccgagggg ctcctaggca
241cagaggggcc cctggaggaa gaagaggatg gagaggagct cattggagat ggcatggaaa
301gggactaccg cgccatccca gagctggacg cctatgaggc cgagggactg gctctggatg
361atgaggacgt agaggagctg acggccagtc agagggaggc agcagagcgg gccatgcggc
421agcgtgaccg ggaggctggc cggggcctgg gccgcatgcg ccgtgggctc ctgtatgaca
481gcgatgagga ggacgaggag cgccctgccc gcaagcgccg ccaggtggag cgggccacgg
541aggacggcga ggaggacgag gagatgattg agagcatcga gaacctggag gatctcaaag
601gccactctgt gcgcgagtgg gtgagcatgg cgggcccccg gctggagatc caccaccgct
661tcaagaactt cctgcgcact cacgtcgaca gccacggcca caacgtcttc aaggagcgca
721tcagcgacat gtgcaaagag aaccgtgaga gcctggtggt gaactatgag gacttggcag
781ccagggagca cgtgctggcc tacttcctgc ctgaggcacc ggcggagctg ctgcagatct
841ttgatgaggc tgccctggag gtggtactgg ccatgtaccc caagtacgac cgcatcacca
901accacatcca tgtccgcatc tcccacctgc ctctggtgga ggagctgcgc tcgctgaggc
961agctgcatct gaaccagctg atccgcacca gtggggtggt gaccagctgc actggcgtcc
1021tgccccagct cagcatggtc aagtacaact gcaacaagtg caatttcgtc ctgggtcctt
1081tctgccagtc ccagaaccag gaggtgaaac caggctcctg tcctgagtgc cagtcggccg
1141gcccctttga ggtcaacatg gaggagacca tctatcagaa ctaccagcgt atccgaatcc
1201aggagagtcc aggcaaagtg gcggctggcc ggctgccccg ctccaaggac gccattctcc
1261tcgcagatct ggtggacagc tgcaagccag gagacgagat agagctgact ggcatctatc
1321acaacaacta tgatggctcc ctcaacactg ccaatggctt ccctgtcttt gccactgtca
1381tcctagccaa ccacgtggcc aagaaggaca acaaggttgc tgtaggggaa ctgaccgatg
1441aagatgtgaa gatgatcact agcctctcca aggatcagca gatcggagag aagatctttg
1501ccagcattgc tccttccatc tatggtcatg aagacatcaa gagaggcctg gctctggccc
1561tgttcggagg ggagcccaaa aacccaggtg gcaagcacaa ggtacgtggt gatatcaacg
1621tgctcttgtg cggagaccct ggcacagcga agtcgcagtt tctcaagtat attgagaaag
1681tgtccagccg agccatcttc accactggcc agggggcgtc ggctgtgggc ctcacggcgt
1741atgtccagcg gcaccctgtc agcagggagt ggaccttgga ggctggggcc ctggttctgg
1801ctgaccgagg agtgtgtctc attgatgaat ttgacaagat gaatgaccag gacagaacca
1861gcatccatga ggccatggag caacagagca tctccatctc gaaggctggc atcgtcacct
1921ccctgcaggc tcgctgcacg gtcattgctg ccgccaaccc cataggaggg cgctacgacc
1981cctcgctgac tttctctgag aacgtggacc tcacagagcc catcatctca cgctttgaca
2041tcctgtgtgt ggtgagggac accgtggacc cagtccagga cgagatgctg gcccgcttcg
2101tggtgggcag ccacgtcaga caccacccca gcaacaagga ggaggagggg ctggccaatg
2161gcagcgctgc tgagcccgcc atgcccaaca cgtatggcgt ggagcccctg ccccaggagg
2221tcctgaagaa gtacatcatc tacgccaagg agagggtcca cccgaagctc aaccagatgg
2281accaggacaa ggtggccaag atgtacagtg acctgaggaa agaatctatg gcgacaggca
2341gcatccccat tacggtgcgg cacatcgagt ccatgatccg catggcggag gcccacgcgc
2401gcatccatct gcgggactat gtgatcgaag acgacgtcaa catggccatc cgcgtgatgc
2461tggagagctt catagacaca cagaagttca gcgtcatgcg cagcatgcgc aagacttttg
2521cccgctacct ttcattccgg cgtgacaaca atgagctgtt gctcttcata ctgaagcagt
2581tagtggcaga gcaggtgaca tatcagcgca accgctttgg ggcccagcag gacactattg
2641aggtccctga gaaggacttg gtggataagg ctcgtcagat caacatccac aacctctcTG
2701CATTTTATGA CAGTGAGCTC TTCAGGATGA ACAAGTTCAG CCACGACCTG AAAAGGAAAA
2761TGATCCTGCA GCAGTTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
2821TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
2881GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATTTATA TGCAAAAGGC
2941AACAAGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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