Construct: ORF TRCN0000466792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013526.1_s317c1
- Derived from:
- ccsbBroadEn_13347
- DNA Barcode:
- GCCCTGTCCCAACGCTGTTGTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC20B (166979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 166979 | CDC20B | cell division cycle 20B | XM_011543218.2 | 89.9% | 89.6% | 64_126del;361C>T |
| 2 | human | 166979 | CDC20B | cell division cycle 20B | NM_001145734.2 | 38.7% | 36.4% | (many diffs) |
| 3 | human | 166979 | CDC20B | cell division cycle 20B | NM_152623.2 | 35.9% | 33.7% | (many diffs) |
| 4 | human | 166979 | CDC20B | cell division cycle 20B | NM_001170402.1 | 35.6% | 33.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtggaaactg gagcgcaccg cgcctcggag ggtccgcacg gaagaggaga 121 tgctgtgggt actcgattca gttaatgcta cgtattctga ctttaagagc aactttgcga 181 agaggctgtc cgcagaggtt cctgttgcga gtagccccat taccacaagg tggcagcaaa 241 gtcaaactag ggctctgtcc tctgattcct ttggggaaga gcagtcaacc acctaccTCC 301 CAGAAGCTTC CGGATCAGTG CTGAAGACAC CGCCTGAGAA AGAGACCTTG ACTCTAGGAT 361 CCTGCAAAGA ACAACTGAAG ACCCCCAGCA AAGGAATTTC TGAAACAAGT AACTCTGCTC 421 TCCATTTTTG CAAGGCACCT CATGCAATGG ACAGAGACTG GAAAGAAAGT GTTGCCTCAA 481 AAGGGCAGAA ATGCCTAAAA CAACTCTTTG TAACCCAGAA CGTGGTTCAG CAGGCTAATG 541 GAAAAATGCA GCTCTGTGAG CAATCCGAAT GTGTTTGGAA AGATCACTTT AGTGGCAGCA 601 TGAAGAAACG ATTTGAACAG GAGGATGTTG GTGGCAAAGA CTACCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAGCC CTGTCCCAAC GCTGTTGTTA AACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t