Construct: ORF TRCN0000466803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007497.1_s317c1
- Derived from:
- ccsbBroadEn_11074
- DNA Barcode:
- GCCATAGCCATTCATCCCGAGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPN9 (5780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466803
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5780 | PTPN9 | protein tyrosine phosphatas... | NM_002833.4 | 98.2% | 97.9% | 1_30del;484A>G;779C>G |
| 2 | mouse | 56294 | Ptpn9 | protein tyrosine phosphatas... | NM_019651.2 | 89.9% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1815
- ORF length:
- 1749
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccggagctg accccggagg aggagcaggc taccaagcag tttctcgaag 121 agattaacaa gtggacagtt cagtacaatg tttccccgct gtcttggaat gtggctgtca 181 agttcctcat ggcaaggaag tttgatgtgc tccgtgccat agaattgttc cactcctaca 241 gagaaactcg aaggaaggaa ggcattgtaa agctgaaacc tcatgaggaa cctcttcgtt 301 ctgagatcct cagtggaaaa ttcaccatct taaatgttcg ggacccaaca ggagcctcca 361 ttgccctctt tactgccagg ttgcatcatc cccacaagtc agtccaacat gtggtacttc 421 aggctctgtt ttacttgcta gacagagctg tggatagctt tgaaactcag aggaatggac 481 tggtgtttat ctatgacatg tgtggttcta attatgccga ctttgagctg gatcttggca 541 agaaagtcct aaacctgctg aagggagcat ttccagctcg tttgaagaag gtgctgattg 601 tgggggcacc catatggttc cgagtgccct attccatcat cagtctcctc ctgaaggaca 661 aagtccggga gaggattcaa atattaaaga catctgaggt cacgcagcat ctgcccaggg 721 agtgtcttcc agaaaacctg ggtgggtacg tcaaaattga tctcgccact tggaatttcc 781 agttcctacc ccaggtgaac ggccacccag atcgcttcga tgagatcatc ctgttctccc 841 tccctcctgc cttagactgg gactcagtac atgttccagg tccccatgct atgaccatcc 901 aagagttggt ggactatgtt aatgccaggc aaaagcaagg aatctatgag gaatatgaag 961 acattcgtcg tgagaaccct gttggcactt tccactgttc catgtctcca ggaaacctag 1021 agaaaaaccg ttatggggat gtaccctgcc tggaccaaac tagagtgaag ctaacaaagc 1081 gaagtggcca tactcagaca gattacatca atgccagttt catggatggc tacaagcaga 1141 agaatgctta cattggcaca caaggtcctt tggagaatac ctatcgtgat ttctggctca 1201 tggtatggga gcaaaaagtc ttggtgattg tcatgaccac ccgctttgag gaaggcggca 1261 ggagaaagtg tggccagtac tggcctttag aaaaagactc tcggatccga tttggcttcc 1321 tcacagtgac caatctaggc gtggagaaca tgaatcatta taagaaaaca acgctagaaa 1381 ttcacaacac agaggaacgg cagaaacgcc aggtgaccca cttccagttc ttgagctggc 1441 cagactatgg tgtcccttcc TCAGCAGCTT CCCTCATTGA CTTCTTGAGA GTGGTCAGAA 1501 ACCAGCAGAG TCTGGCTGTG AGCAACATGG GAGCACGCTC CAAAGGGCAG TGCCCTGAGC 1561 CACCCATTGT GGTCCATTGC AGTGCAGGCA TTGGCAGGAC AGGTACCTTC TGCTCACTGG 1621 ACATCTGCCT GGCACAGCTG GAGGAGCTTG GCACCCTTAA TGTGTTCCAG ACGGTGTCAC 1681 GCATGAGGAC CCAGAGGGCC TTCAGCATCC AGACCCCTGA GCAGTACTAT TTTTGCTACA 1741 AGGCCATCCT GGAGTTCGCA GAGAAGGAGG GCATGGTATC CTCTGGCCAA AACCTGCTGG 1801 CCGTGGAGAG TCAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1861 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1921 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCCATAGCCA TTCATCCCGA 1981 GCTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt