Construct: ORF TRCN0000466822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008218.1_s317c1
- Derived from:
- ccsbBroadEn_05530
- DNA Barcode:
- TTCCGTCATGCTACGGATAAGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTK1 (373156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466822
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 373156 | GSTK1 | glutathione S-transferase k... | NM_001143679.1 | 100% | 100% | |
| 2 | human | 373156 | GSTK1 | glutathione S-transferase k... | NM_015917.3 | 80.1% | 80.1% | 383_384ins168 |
| 3 | human | 373156 | GSTK1 | glutathione S-transferase k... | NM_001143680.1 | 75.8% | 75.8% | 383_384ins204 |
| 4 | human | 373156 | GSTK1 | glutathione S-transferase k... | NM_001143681.1 | 64.8% | 64.8% | 153_154ins129;254_255ins168 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 912
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcccctgccg cgcaccgtgg agctcttcta tgacgtgctg tccccctact 121 cctggctggg cttcgagatc ctgtgccggt atcagaatat ctggaacatc aacctgcagt 181 tgcggcccag cctcataaca gggatcatga aagacagtgg aaacaagcct ccaggtctgc 241 ttccccgcaa aggactatac atggcaaatg acttaaagct cctgagacac catctccaga 301 ttcccatcca cttccccaag gatttcttgt ctgtgatgct tgaaaaagga agtttgtctg 361 ccatgcgttt cctcaccgcc gtgaacttgg agcatccaga gatgctggag aaagcgtccc 421 gggagctgtg gatgcgcgtc tggtcaaggg tgagtgtggg gctctgggaa tcctctggga 481 ggaccttgga tgactttctg accttcccca ggcacgtttt CAGGGTCATG ATCCTGCCCC 541 CGCCCGGGGG ATCTACTGTC CTCCCAGTCA CACCCCTCTC CCCGCACCGC CTTCCTGCTG 601 TCTTCTCTTC TTCCCAGAAT GAAGACATCA CCGAGCCGCA GAGCATCCTG GCGGCTGCAG 661 AGAAGGCTGG TATGTCTGCA GAACAAGCCC AGGGACTTCT GGAAAAGATC GCAACGCCAA 721 AGGTGAAGAA CCAGCTCAAG GAGACCACTG AGGCAGCCTG CAGATACGGA GCCTTTGGGC 781 TGCCCATCAC CGTGGCCCAT GTGGATGGCC AAACCCACAT GTTATTTGGC TCTGACCGGA 841 TGGAGCTGCT GGCGCACCTG CTGGGAGAGA AGTGGATGGG CCCTATACCT CCAGCCGTGA 901 ATGCCAGACT TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 961 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1021 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC CGTCATGCTA CGGATAAGAC 1081 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t