Construct: ORF TRCN0000466864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011722.1_s317c1
- Derived from:
- ccsbBroadEn_00734
- DNA Barcode:
- TCACAGTTGCTGCGACATACGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDAC1 (3065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466864
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3065 | HDAC1 | histone deacetylase 1 | NM_004964.3 | 100% | 100% | |
| 2 | human | 3065 | HDAC1 | histone deacetylase 1 | XM_011541309.2 | 59.9% | 59.9% | 0_1ins579 |
| 3 | mouse | 433759 | Hdac1 | histone deacetylase 1 | NM_008228.2 | 90.4% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1512
- ORF length:
- 1446
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagacgcag ggcacccgga ggaaagtctg ttactactac gacggggatg 121 ttggaaatta ctattatgga caaggccacc caatgaagcc tcaccgaatc cgcatgactc 181 ataatttgct gctcaactat ggtctctacc gaaaaatgga aatctatcgc cctcacaaag 241 ccaatgctga ggagatgacc aagtaccaca gcgatgacta cattaaattc ttgcgctcca 301 tccgtccaga taacatgtcg gagtacagca agcagatgca gagattcaac gttggtgagg 361 actgtccagt attcgatggc ctgtttgagt tctgtcagtt gtctactggt ggttctgtgg 421 caagtgctgt gaaacttaat aagcagcaga cggacatcgc tgtgaattgg gctgggggcc 481 tgcaccatgc aaagaagtcc gaggcatctg gcttctgtta cgtcaatgat atcgtcttgg 541 ccatcctgga actgctaaag tatcaccaga gggtgctgta cattgacatt gatattcacc 601 atggtgacgg cgtggaagag gccttctaca ccacggaccg ggtcatgact gtgtcctttc 661 ataagtatgg agagtacttc ccaggaactg gggacctacg ggatatcggg gctggcaaag 721 gcaagtatta tgctgttaac tacccgctcc gagacgggat tgatgacgag tcctatgagg 781 ccattttcaa gccggtcatg tccaaagtaa tggagatgtt ccagcctagt gcggtggtct 841 tacagtgtgg ctcagactcc ctatctgggg atcggttagg ttgcttcaat ctaactatca 901 aaggacacgc caagtgtgtg gaatttgtca agagctttaa cctgcctatg ctgatgctgg 961 gaggcggtgg ttacaccatt cgtaacgttg cccggtgctg gacatatgag acagctgtgg 1021 ccctggatac ggagatccct aatgagcttc catacaatga ctactttgaa tactttggac 1081 cagatttcaa gctccacatc agtccttcca atatgactaa ccagaacacg aatgagtacc 1141 tggagaagat caaacagcga ctgtttgaga accttagaat gctgccgcac gcacctgggg 1201 tccaaatgca ggcgattcct gaggacgcca tccctgagga gagtggcgat gaggacgaag 1261 acgaccctga caagcgcatc tcgatctgct cctctgacaa acgaattgcc tgtgaggaag 1321 agttctccga ttctgaagag gagggagagg ggggccgcaa gaactcttcc aacttcaaaa 1381 aagccaagag agtcaaaaca gaggatgaaa aagagaaaga cccagaggag aagaaagaag 1441 tcaccgaaga ggagaaaacc aagGAGGAGA AGCCAGAAGC CAAAGGGGTC AAGGAGGAGG 1501 TCAAGTTGGC CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA CAGTTGCTGC GACATACGAA 1681 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t