Construct: ORF TRCN0000466891
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001868.1_s317c1
- Derived from:
- ccsbBroadEn_11385
- DNA Barcode:
- GCTGCCCTGCGGATGCATCATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TP53I11 (9537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466891
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9537 | TP53I11 | tumor protein p53 inducible... | NR_134612.2 | 10.4% | 1_2347del;2711_3475del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cacaccggaa gtggcgagcc caagccctgg ggacaggtgt agggagaaaa 121 gcagccccag gccTCAGACT CGCTTCCCAT CCTGGCATAG AGTGGGGGAT GGCTGGAGGG 181 TGTCTATAGG TACAGCCCGC TCTGGCTGCT GCCAGGTGGG CCCCTGCCAG GGGTCCTCAC 241 CCCTGTCCAC CCTGTGCCTG GCTGTCCCTG CACCCAGATA CAGCAACATG GCCTGTACCC 301 AGCAGAGTGG TGGCACCACC ATGGTTACAG CGGATGCCCC GAGACTCTGC TTGGTAAACG 361 TGGCAGAGCA GAATGGGAGG CTGGACCCTG AGGAAGGGCC CCTCTCCTGG CATCTGTCTC 421 TTGCTACCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCTGCC CTGCGGATGC ATCATGTCAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt