Construct: ORF TRCN0000466896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008106.1_s317c1
- Derived from:
- ccsbBroadEn_10848
- DNA Barcode:
- GGACCGACAGCTTCCTTACACCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLUD2 (2747)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466896
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318901.1 | 66.4% | 65.2% | (many diffs) |
| 2 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318902.1 | 66.4% | 65.2% | (many diffs) |
| 3 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318904.2 | 66.4% | 65.2% | (many diffs) |
| 4 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318905.2 | 66.4% | 65.2% | (many diffs) |
| 5 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318906.2 | 66.4% | 65.2% | (many diffs) |
| 6 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_001318900.1 | 61% | 60% | (many diffs) |
| 7 | human | 2747 | GLUD2 | glutamate dehydrogenase 2 | NM_012084.4 | 47.3% | 47.3% | 1_882del |
| 8 | human | 2746 | GLUD1 | glutamate dehydrogenase 1 | NM_005271.5 | 46.5% | 45.6% | (many diffs) |
| 9 | mouse | 14661 | Glud1 | glutamate dehydrogenase 1 | NM_008133.4 | 43.6% | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac accagggttt agagataaaa catttgttgt tcagggattt ggtaatgtgg 121 gcctacactc tatgagatat ttacatcgtt ttggtgctaa atgtattgct gttggtgagt 181 ctgatgggag tatatggaat ccagatggta ttgacccaaa ggaactggaa gacttcaaat 241 tgcaacatgg gtccattctg ggcttcccca aggcaaagcc ctatgaagga agcatcttgg 301 aggtcgactg tgacatactg atcccagctg ccactgagaa gcagttgacc aaatccaacg 361 cacccagagt caaagccaag atcattgctg aaggtgccaa tgggccaaca actccagaag 421 ctgataagat cttcctggag agaaacattt tggttattcc agatctctac ttgaatgctg 481 gaggagtgac agtatcttac tttgagtggc tgaagaatct aaatcatgtc agctatggcc 541 gtttgacctt caaatatgaa agggattcta actaccactt gcTCCTGTCT GTTCAAGAGA 601 GTTTAGAAAG AAAATTTGGA AAGCATGGTG GAACTATTCC CATTGTACCC ACGGCAGAGT 661 TCCAAGACAG TATATCGGGT GCATCTGAGA AAGACATTGT GCACTCTGCC TTGGCATACA 721 CAATGGAGCG TTCTGCCAGG CAAATTATGC ACACAGCCAT GAAGTATAAC CTGGGATTGG 781 ACCTGAGAAC AGCTGCCTAT GTCAATGCCA TTGAAAAAGT CTTCAAAGTG TACAGTGAAG 841 CTGGTGTGAC CTTCACATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAG 961 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGACCGAC AGCTTCCTTA 1021 CACCTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt