Construct: ORF TRCN0000466924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015914.1_s317c1
- Derived from:
- ccsbBroadEn_06423
- DNA Barcode:
- TAGCATTTGAACGATATGTGGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IDH3G (3421)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3421 | IDH3G | isocitrate dehydrogenase (N... | NM_004135.4 | 99.9% | 99.7% | 799T>C |
| 2 | human | 3421 | IDH3G | isocitrate dehydrogenase (N... | NM_174869.3 | 93.8% | 88.4% | (many diffs) |
| 3 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | NM_008323.1 | 87.8% | 94.9% | (many diffs) |
| 4 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | XM_006527843.3 | 86.8% | 94.1% | (many diffs) |
| 5 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | XM_006527844.1 | 76% | 83.4% | (many diffs) |
| 6 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | XM_017318392.1 | 76% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1245
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgaaggta gcgaccgtcg ccggcagcgc cgcgaaggcg gtgctcgggc 121 cagcccttct ctgccgtccc tgggaggttc taggcgccca cgaggtcccc tcgaggaaca 181 tcttttcaga acaaacaatt cctccgtccg ctaagtatgg cgggcggcac acggtgacca 241 tgatcccagg ggatggcatc gggccagagc tcatgctgca tgtcaagtcc gtcttcaggc 301 acgcatgtgt accagtggac tttgaagagg tgcacgtgag ttccaatgct gatgaagagg 361 acattcgcaa tgccatcatg gccatccgcc ggaaccgcgt ggccctgaag ggcaacatcg 421 aaaccaacca taacctgcca ccgtcgcaca aatctcgaaa caacatcctt cgcaccagcc 481 tggacctcta tgccaacgtc atccactgta agagccttcc aggcgtggtg acccggcaca 541 aggacataga catcctcatt gtccgggaga acacagaggg cgagtacagc agcctggagc 601 atgagagtgt ggcgggagtg gtggagagcc tgaagatcat caccaaggcc aagtccctgc 661 gcattgccga gtatgccttc aagctggcgc aggagagcgg gcgcaagaaa gtgacggccg 721 tgcacaaggc caacatcatg aaactgggcg atgggctttt cctccagtgc tgcagggagg 781 tggcagcccg ctaccctcag atcaccTTCG AGAACATGAT TGTGGATAAC ACCACCATGC 841 AGCTGGTGTC CCGGCCCCAG CAGCTTGATG TCATGGTGAT GCCCAATCTC TATGGCAACA 901 TCGTCAACAA TGTCTGCGCG GGACTGGTCG GGGGCCCAGG CCTTGTGGCT GGGGCCAACT 961 ATGGCCATGT GTACGCGGTG TTTGAAACAG CTACGAGGAA CACCGGCAAG AGTATCGCCA 1021 ATAAGAACAT CGCCAACCCC ACGGCCACCC TGCTGGCCAG CTGCATGATG CTGGACCACC 1081 TCAAGCTGCA CTCCTATGCC ACCTCCATCC GTAAGGCTGT CCTGGCATCC ATGGACAATG 1141 AGAATATGCA CACTCCGGAC ATCGGGGGCC AGGGCACAAC ATCTGAAGCC ATCCAGGACG 1201 TCATCCGCCA CATCCGCGTC ATCAACGGCC GGGCCGTGGA GGCCTACCCA ACTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA TAGCATTTGA ACGATATGTG GGTCACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt