Construct: ORF TRCN0000466990
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000063.1_s317c1
- Derived from:
- ccsbBroadEn_15168
- DNA Barcode:
- TAGACGGTTTTATACGCTCAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFHD1 (80303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466990
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80303 | EFHD1 | EF-hand domain family membe... | NM_025202.4 | 100% | 100% | |
| 2 | human | 80303 | EFHD1 | EF-hand domain family membe... | NM_001243252.1 | 59.4% | 58.3% | (many diffs) |
| 3 | human | 80303 | EFHD1 | EF-hand domain family membe... | NM_001308395.2 | 53.1% | 53.1% | 0_1ins336 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagtgaggag ctggcgtgca agctggagcg ccggctgcgg cgcgaggagg 121 ccgaggagag tggcccccag ctggctcccc tcggcgcccc agccccggag cccaagcccg 181 agcccgagcc tcccgcccgt gcgcccacgg ccagcgccga cgcggagctg agcgcccagc 241 tgagccggcg gctggacatc aacgagggcg ctgcgcggcc ccggcgctgc agggtcttca 301 acccctacac ggagttcccg gagttcagcc gccgcctcat caaggacctg gagagcatgt 361 tcaaactgta tgacgctggg cgggatggct tcatcgaccT GATGGAGCTG AAGCTGATGA 421 TGGAGAAGCT GGGGGCCCCC CAGACCCACC TGGGCCTGAA GAGCATGATC AAGGAGGTGG 481 ATGAGGACTT CGATGGCAAG CTCAGCTTCC GGGAGTTCCT GCTCATTTTC CACAAGGCCG 541 CGGCAGGGGA GCTGCAGGAG GACAGTGGGC TGATGGCGCT GGCAAAGCTT TCTGAGATCG 601 ATGTGGCCCT GGAGGGTGTC AAAGGTGCCA AGAACTTCTT TGAAGCCAAG GTCCAAGCCT 661 TGTCATCGGC CAGTAAGTTT GAAGCAGAGT TGAAAGCTGA GCAAGATGAG CGGAAGCGGG 721 AGGAGGAGGA GAGGCGGCTC CGCCAGGCAG CCTTCCAGAA ACTCAAGGCC AACTTCAATA 781 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAGTATTT CGATTTCTTG 901 GCTTTATATA TCTTGTGGAA AGGACGATAG ACGGTTTTAT ACGCTCAGCC AACGCGTTAA 961 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t