Construct: ORF TRCN0000467070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011855.1_s317c1
- Derived from:
- ccsbBroadEn_10293
- DNA Barcode:
- CGTCCTCTCCCGCCCGCTTAGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFL1P1 (142913)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467070
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 142913 | CFL1P1 | cofilin 1 pseudogene 1 | NR_028492.1 | 15.5% | 1_377del;711_2141del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 423
- ORF length:
- 333
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGCTCCCA TGTGGCTGTC TTTGATGATG 121 TCATCAAGGT GTTCAGTGAC ATGAAAGTGT GCAAGTCTTC AACACTAGAA AAGGTGAAGA 181 AGCGCAAGAA GTTGCTGCTC TTCTGCCTGA GTGAGTACAA GAAGTACATC ATCCTTGCGG 241 AGGCCAAGAA GATCCTGGTA AGTAATGTGG ACCAAACCAT TGATGATCCC TATGCCACTT 301 TTGTCAAGAT GCTGACAGTA AGGACTGCCG CTACGCCCCT TATGACGCCA CCAAGGACAG 361 CAAGAAGAAG GACCTGGTGT TTATCTTCTG GGCCTCTGAG TCTGCATCCA TTAAGAGCAA 421 AATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGACG TCCTCTCCCG CCCGCTTAGC AGACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt