Construct: ORF TRCN0000467247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000750.1_s317c1
- Derived from:
- ccsbBroadEn_16120
- DNA Barcode:
- AAAACCTTTGAGCGGAGCAAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPTSSA (171546)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467247
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 171546 | SPTSSA | serine palmitoyltransferase... | NM_138288.4 | 95.7% | 95.7% | 1_9delATGGCGGGG |
| 2 | mouse | 104725 | Sptssa | serine palmitoyltransferase... | NM_134054.2 | 87.3% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 221
- ORF end:
- 425
- ORF length:
- 204
- Sequence:
-
1 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA 61 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC 121 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC 181 TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGCGCTGG CGCGGGCCTG 241 GAAGCAGATG TCCTGGTTCT ACTACCAGTA CCTGCTGGTC ACGGCGCTCT ACATGCTGGA 301 GCCCTGGGAG CGGACGGTGT TCAATTCCAT GCTGGTTTCC ATTGTGGGGA TGGCACTATA 361 CACAGGATAC GTCTTCATGC CCCAGCACAT CATGGCGATA TTGCACTACT TTGAAATCGT 421 ACAATGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 481 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 541 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAAACCTTTG AGCGGAGCAA ATATACGCGT 601 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt