Construct: ORF TRCN0000467363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001395.1_s317c1
- Derived from:
- ccsbBroadEn_06174
- DNA Barcode:
- CGCTTAACAAGTTTTGGTGCAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERCC1 (2067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467363
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369412.1 | 99.8% | 100% | 354T>C |
| 2 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369413.1 | 99.8% | 100% | 354T>C |
| 3 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369414.1 | 99.8% | 100% | 354T>C |
| 4 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369415.1 | 99.8% | 100% | 354T>C |
| 5 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369416.1 | 99.8% | 100% | 354T>C |
| 6 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001983.4 | 99.8% | 100% | 354T>C |
| 7 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001166049.2 | 91.8% | 91.9% | 354T>C;702_703ins72 |
| 8 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369417.1 | 91.8% | 91.9% | 354T>C;702_703ins72 |
| 9 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369418.1 | 91.8% | 91.9% | 354T>C;702_703ins72 |
| 10 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369419.1 | 91.8% | 91.9% | 354T>C;702_703ins72 |
| 11 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369408.1 | 86.4% | 86.1% | (many diffs) |
| 12 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369409.1 | 86.4% | 86.1% | (many diffs) |
| 13 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_202001.3 | 86.4% | 86.1% | (many diffs) |
| 14 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369410.1 | 79.1% | 78.8% | (many diffs) |
| 15 | human | 2067 | ERCC1 | ERCC excision repair 1, end... | NM_001369411.1 | 79.1% | 78.8% | (many diffs) |
| 16 | mouse | 13870 | Ercc1 | excision repair cross-compl... | NM_007948.2 | 82.2% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccctgggaag gacaaagagg gggtgcccca gccctcaggg ccgccagcaa 121 ggaagaaatt tgtgataccc ctcgacgagg atgaggtccc tcctggagtg gccaagccct 181 tattccgatc tacacagagc cttcccactg tggacacctc ggcccaggcg gcccctcaga 241 cctacgccga atatgccatc tcacagcctc tggaaggggc tggggccacg tgccccacag 301 ggtcagagcc cctggcagga gagacgccca accaggccct gaaacccggg gcaaaatcca 361 acagcatcat tgtgagccct cggcagaggg gcaatcccgt actgaagttc gtgcgcaacg 421 tgccctggga atttggcgac gtaattcccg actatgtgct gggccagagc acctgtgccc 481 tgttcctcag cctccgctac cacaacctgc acccagacta catccatggg cggctgcaga 541 gcctggggaa gaacttcgcc ttgcgggtcc tgcttgtcca ggtggatgtg aaagatcccc 601 agcaggccct caaggagctg gctaagatgt gtatcctggc cgactgcaca ttgaTCCTCG 661 CCTGGAGCCC CGAGGAAGCT GGGCGGTACC TGGAGACCTA CAAGGCCTAT GAGCAGAAAC 721 CAGCGGACCT CCTGATGGAG AAGCTAGAGC AGGACTTCGT CTCCCGGGTG ACTGAATGTC 781 TGACCACCGT GAAGTCAGTC AACAAAACGG ACAGTCAGAC CCTCCTGACC ACATTTGGAT 841 CTCTGGAACA GCTCATCGCC GCATCAAGAG AAGATCTGGC CTTATGCCCA GGCCTGGGCC 901 CTCAGAAAGC CCGGAGGCTG TTTGATGTCC TGCACGAGCC CTTCTTGAAA GTACCCTGCC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GACGCTTAAC AAGTTTTGGT GCAGTTACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt