Construct: ORF TRCN0000467556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005779.1_s317c1
- Derived from:
- ccsbBroadEn_12449
- DNA Barcode:
- CACCATGACGTAACGTTTATGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRL4 (64123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64123 | ADGRL4 | adhesion G protein-coupled ... | NM_022159.4 | 87.7% | 87.6% | 1_252del;898G>T;1482C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1884
- ORF length:
- 1818
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgtacctggc ttcagatcca gcagtaacca agacaggttt atcactaatg 121 atggaaccgt ctgtatagaa aatgtgaatg caaactgcca tttagataat gtctgtatag 181 ctgcaaatat taataaaact ttaacaaaaa tcagatccat aaaagaacct gtggctttgc 241 tacaagaagt ctatagaaat tctgtgacag atctttcacc aacagatata attacatata 301 tagaaatatt agctgaatca tcttcattac taggttacaa gaacaacact atctcagcca 361 aggacaccct ttctaactca actcttactg aatttgtaaa aaccgtgaat aattttgttc 421 aaagggatac atttgtagtt tgggacaagt tatctgtgaa tcataggaga acacatctta 481 caaaactcat gcacactgtt gaacaagcta ctttaaggat atcccagagc ttccaaaaga 541 ccacagagtt tgatacaaat tcaacggata tagctctcaa agttttcttt tttgattcat 601 ataacatgaa acatattcat cctcatatga atatggatgg agactacata aatatatttc 661 caaagagaaa agctgcatat gattcaaatg gcaatgttgc agttgcattt ttatattata 721 agagtattgg tcctttgctt tcatcatctg acaacttctt attgaaacct caaaattatg 781 ataattctga agaggaggaa agagtcatat cttcagtaat ttcagtctca atgagctcaa 841 acccacccac attatatgaa cttgaaaaaa taacatttac attaagtcat cgaaaggtca 901 cagataggta taggagtcta tgtgcatttt ggaattactc acctgatacc atgaatggca 961 gctggtcttc agagggctgt gagctgacat actcaaatga gacccacacc tcatgccgct 1021 gtaatcacct gacacatttt gcaattttga tgtcctctgg tccttccatt ggtattaaag 1081 attataatat tcttacaagg atcactcaac taggaataat tatttcactg atttgtcttg 1141 ccatatgcat ttttaccttc tggttcttca gtgaaattca aagcaccagg acaacaattc 1201 acaaaaatct ttgctgtagc ctatttcttg ctgaacttgt ttttcttgtt gggatcaata 1261 caaatactaa taagctcttc tgttcaatca ttgctggact gctacactac ttctttttag 1321 ctgcttttgc atggatgtgc attgaaggca tacatctcta tctcattgtt gtgggtgtca 1381 tctacaacaa gggatttttg cacaagaatt tttatatctt tggctatcta agcccagccg 1441 tggtagttgg attttcggca gcactaggat acagatatta tggcacaacc aaagtatgtt 1501 ggcttagcac cgaaaacaac tttatttgga gttttatagg accagcatgc ctaatcattc 1561 ttgttaatct cttggctttt GGAGTCATCA TATACAAAGT TTTTCGTCAC ACTGCAGGGT 1621 TGAAACCAGA AGTTAGTTGC TTTGAGAACA TAAGGTCTTG TGCAAGAGGA GCCCTCGCTC 1681 TTCTGTTCCT TCTCGGCACC ACCTGGATCT TTGGGGTTCT CCATGTTGTG CACGCATCAG 1741 TGGTTACAGC TTACCTCTTC ACAGTCAGCA ATGCTTTCCA GGGGATGTTC ATTTTTTTAT 1801 TCCTGTGTGT TTTATCTAGA AAGATTCAAG AAGAATATTA CAGATTGTTC AAAAATGTCC 1861 CCTGTTGTTT TGGATGTTTA AGGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1921 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1981 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ACCATGACGT 2041 AACGTTTATG TAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2101 att