Construct: ORF TRCN0000467562
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014438.1_s317c1
- Derived from:
- ccsbBroadEn_11233
- DNA Barcode:
- ACAATCGAAATTCTGACTGCTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF99 (7652)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467562
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105371063 | LOC105371063 | putative uncharacterized pr... | XM_011522781.1 | 28.4% | 7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaatgtggca aacactgtaa ccatttctca catcttacca cacattaaga 121 taattcatac tgaagagaaa cccgacaaat gtgaagaatg taacaaagtc tttaactggt 181 cttcaactca tactaaatat aagagaattc atacagaaga taaattttat aaatatgaag 241 aatgtgacaa aagctttaaa aacatttcaa cccttattac acataagata atttatgttg 301 tagagaaatt ctacaaatgt gaagaatgtg gcaaagtttt tttttttttt ttgaaatgga 361 gtctcactct gtcgcccaaa ttggagtgca atggtgcaat ctcagttcat tgcaacctcc 421 gcctcctggg ttcaagcgat tctctggcct caaccTCCCA AGCAGCTGGG ATTGCAGGCG 481 CCTGCCACCA CGCCCAGCTA ATTTTTGTAT TTTTAGTAGA GACGGGGTTT CACCATTTTG 541 ACCAGGCTGG TTTTGAACTC CTGACCTCAA GTGATCCACC CGCCTTGGCC TCCCAAAGTG 601 CCCCAAAGTG CTGGGATTAC AAGCATGAGC CATTGAGCCC GGTCGAATGT GGCAAAGTTT 661 TTAATAAGTT ATCAAATCAT ACTGGAGAGA AGCTCTACAA ACCTAAAAGA CATGACAGTG 721 CTCTTGAGAA CACCTTAAAC TTTTCTAAAC ATAAAAGAAA TCATAGTGTC AAGAAGCCCT 781 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 901 TTTATATATC TTGTGGAAAG GACGAACAAT CGAAATTCTG ACTGCTCTCA CGCGTTAAGT 961 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt