Construct: ORF TRCN0000467636
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007460.1_s317c1
- Derived from:
- ccsbBroadEn_08956
- DNA Barcode:
- TCGGCGGAATAACCCAAGGCACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TXNDC15 (79770)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467636
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79770 | TXNDC15 | thioredoxin domain containi... | NM_024715.3 | 99.9% | 100% | 162G>T |
| 2 | human | 79770 | TXNDC15 | thioredoxin domain containi... | NM_001350735.1 | 81.1% | 81.1% | 0_1ins204 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaanaag 61 ttggcatggt cccggctgcc ggtcgacgac cgccccgcgt catgcggctc ctcggctggt 121 ggcaagtatt gctgtgggtg ctgggacttc ccgtccgcgg cgtggaggtt gcagaggaaa 181 gtggtcgctt atggtcagag gagcagcctg ctcaccctct ccaggttggg gctgtgtacc 241 tgggtgagga ggagctcctg catgacccga tgggccagga cagggcagca gaagaggcca 301 atgcggtgct ggggctggac acccaaggcg atcacatggt gatgctgtct gtgattcctg 361 gggaagctga ggacaaagtg agttcagagc ctagcggcgt cacctgtggt gctggaggag 421 cggaggactc aaggtgcaac gtccgagaga gccttttctc tctggatggc gctggagcac 481 acttccctga cagagaagag gagtattaca cagagccaga agtggcggaa tctgacgcag 541 ccccgacaga ggactccaat aacactgaaa gtctgaaatc cccaaaggtg aactgtgagg 601 agagaaacat tacaggatta gaaaatttca ctctgaaaat tttaaatatg tcacaggacc 661 ttatggattt tctgaaccca aacggtagtg actgtactct agtcctgttt tacaccccgt 721 ggtgccgctt ttctgccagt ttggcccctc actttaactc tctgccccgg gcatttccag 781 ctcttcactt tttggcactg gatgcatctc agcacagcag cctttctacc aggtttGGCA 841 CCGTAGCTGT TCCTAATATT TTATTATTTC AAGGAGCTAA ACCAATGGCC AGATTTAATC 901 ATACAGATCG AACACTGGAA ACACTGAAAA TCTTCATTTT TAATCAGACA GGTATAGAAG 961 CCAAGAAGAA TGTGGTGGTA ACTCAAGCCG ACCAAATAGG CCCTCTTCCC AGCACTTTGA 1021 TAAAAAGTGT GGACTGGTTG CTTGTATTTT CCTTATTCTT TTTAATTAGT TTTATTATGT 1081 ATGCTACCAT TCGAACTGAG AGTATTCGGT GGCTAATTCC AGGACAAGAG CAGGAACATG 1141 TGGAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCGGCGGAA TAACCCAAGG CACAAACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt