Construct: ORF TRCN0000467717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008148.1_s317c1
- Derived from:
- ccsbBroadEn_05487
- DNA Barcode:
- CATCGCTCACGCATCTGCCAAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NANOS3 (342977)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 342977 | NANOS3 | nanos C2HC-type zinc finger 3 | NM_001098622.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 645
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggaccttt gacctgtgga cagattacct gggtttggca cacctggtta 121 gggctctgag tgggaaagag ggtcctgaaa ccaggctgag cccccagcca gagccagagc 181 caatgctgga gccggtgtca gccctggagc cgatgccagc gccGGAGTCG GTGCCAGTGC 241 CGGGACCCAA GGATCAGAAG CGCAGCCTGG AGTCCTCGCC AGCTCCCGAA CGCCTGTGCT 301 CTTTCTGCAA ACACAACGGC GAGTCCCGGG CCATCTACCA GTCCCACGTG CTGAAGGACG 361 AGGCTGGCAG GGTGCTGTGT CCCATCCTGC GGGACTACGT GTGTCCCCAG TGCGGCGCCA 421 CACGTGAGCG CGCCCACACC CGACGCTTCT GCCCACTTAC TGGCCAGGGC TACACCTCCG 481 TCTACAGCCA CACCACCCGA AACTCGGCAG GCAAGAAGCT GGTCCGGCCT GACAAGGCGA 541 AGACACAGGA CACAGGCCAC CGCCGAGGAG GAGGAGGAGG AGCAGGTTTC AGAGGTGCCG 601 GGAAGTCTGA GCCTTCGCCC TCCTGCTCTC CCTCCATGTC CACCTTGCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA CATCGCTCAC GCATCTGCCA AGGTACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt