Construct: ORF TRCN0000467733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006679.1_s317c1
- Derived from:
- ccsbBroadEn_01652
- DNA Barcode:
- TGCATTGAAGGTCGTATGATGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCN2 (6948)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467733
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6948 | TCN2 | transcobalamin 2 | NM_001184726.1 | 100% | 100% | |
| 2 | human | 6948 | TCN2 | transcobalamin 2 | NM_000355.4 | 93.6% | 93.6% | 347_427del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcaccttggg gccttcctct tccttctggg ggtcctgggg gccctcactg 121 agatgtgtga aataccagag atggacagcc atctggtaga gaagttgggc cagcacctct 181 taccttggat ggaccggctt tccctggagc acttgaaccc cagcatctat gtgggcctac 241 gcctctccag tctgcaggct gggaccaagg aagacctcta cctgcacagc ctcaagcttg 301 gttaccagca gtgcctccta gggtctgcct tcagcgagga tgacggtgac tgccagggca 361 agccttccat gggccagctg gccctctacc tgctcgctct cagagccaac tggcatgatc 421 acaagggcca cccccacact agctactacc agtatggcct gggcattctg gccctgtgtc 481 tccaccagaa gcgggtccat gacagcgtgg tggacaaact tctgtatgct gtggaacctt 541 tccaccaggg ccaccattct gtggacacag cagccatggc aggcttggca ttcacctgtc 601 tgaagcgctc aaacttcaac cctggtcgga gacaacggat caccatggcc atcagaacag 661 tgcgagagga gatcttgaag gcccagaccc ccgagggcca ctttgggaat gtctacagca 721 ccccattggc attacagttc ctcatgactt cccccatgcg tggggcagaa ctgggaacag 781 catgtctcaa ggcgagggtt gctttgctgg ccagtctgca ggatggagcc ttccagaatg 841 ctctcatgat ttcccagctg ctgcccgttc tgaaccacaa gacctacatt gatctgatct 901 tcccagactg tctggcacca cgagtcatgt tggaaccagc tgctgagacc attccTCAGA 961 CCCAAGAGAT CATCAGTGTC ACGCTGCAGG TGCTTAGTCT CTTGCCGCCG TACAGACAGT 1021 CCATCTCTGT TCTGGCCGGG TCCACCGTGG AAGATGTCCT GAAGAAGGCC CATGAGTTAG 1081 GAGGATTCAC ATATGAAACA CAGGCCTCCT TGTCAGGCCC CTACTTAACC TCCGTGATGG 1141 GGAAAGCGGC CGGAGAAAGG GAGTTCTGGC AGCTTCTCCG AGACCCCAAC ACCCCACTGT 1201 TGCAAGGTAT TGCTGACTAC AGACCCAAGG ATGGAGAAAC CATTGAGCTG AGGCTGGTTA 1261 GCTGGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGCATTGAA GGTCGTATGA TGGGTACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt