Construct: ORF TRCN0000467777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000627.1_s317c1
- Derived from:
- ccsbBroadEn_06286
- DNA Barcode:
- AATGGAGCAAGTGACGGTTGAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLUL (2752)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2752 | GLUL | glutamate-ammonia ligase | NM_001033044.4 | 99.9% | 100% | 195T>C |
| 2 | human | 2752 | GLUL | glutamate-ammonia ligase | NM_001033056.3 | 99.9% | 100% | 195T>C |
| 3 | human | 2752 | GLUL | glutamate-ammonia ligase | NM_002065.6 | 99.9% | 100% | 195T>C |
| 4 | human | 2752 | GLUL | glutamate-ammonia ligase | XM_006711278.2 | 99.9% | 100% | 195T>C |
| 5 | mouse | 14645 | Glul | glutamate-ammonia ligase (g... | NM_008131.4 | 89.3% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cacctcagca agttcccact taaataaagg catcaagcag gtgtacatgt 121 ccctgcctca gggtgagaaa gtccaggcca tgtatatctg gatcgatggt actggagaag 181 gactgcgctg caagacccgg accctggaca gtgagcccaa gtgtgtggaa gagttgcctg 241 agtggaattt cgatggctcc agtactttac agtctgaggg ttccaacagt gacatgtatc 301 tcgtgcctgc tgccatgttt cgggacccct tccgtaagga ccctaacaag ctggtgttat 361 gtgaagtttt caagtacaat cgaaggcctg cagagaccaa tttgaggcac acctgtaaac 421 ggataatgga catggtgagc aaccagcacc cctggtttgg catggagcag gagtataccc 481 tcatggggac agatgggcac ccctttggtt ggccttccaa cggcttccca gggccccagg 541 gtccatatta ctgtggtgtg ggagcagaca gagcctatgg cagggacatc gtggaggccc 601 attaccgggc ctgcttgtat gctggagtca agattgcggg gactaatgcc gaggtcatgc 661 ctgcccagtg ggaatttcag attggacctt gtgaaggaat cagcatggga gatcatctct 721 gggtggcccg tttcatcttg catcgtgtgt gtgaagactt tGGAGTGATA GCAACCTTTG 781 ATCCTAAGCC CATTCCTGGG AACTGGAATG GTGCAGGCTG CCATACCAAC TTCAGCACCA 841 AGGCCATGCG GGAGGAGAAT GGTCTGAAGT ACATCGAGGA GGCCATTGAG AAACTAAGCA 901 AGCGGCACCA GTACCACATC CGTGCCTATG ATCCCAAGGG AGGCCTGGAC AATGCCCGAC 961 GTCTAACTGG ATTCCATGAA ACCTCCAACA TCAACGACTT TTCTGCTGGT GTAGCCAATC 1021 GTAGCGCCAG CATACGCATT CCCCGGACTG TTGGCCAGGA GAAGAAGGGT TACTTTGAAG 1081 ATCGTCGCCC CTCTGCCAAC TGCGACCCCT TTTCGGTGAC AGAAGCCCTC ATCCGCACGT 1141 GTCTTCTCAA TGAAACCGGC GATGAGCCCT TCCAGTACAA AAATTACCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA AATGGAGCAA GTGACGGTTG AGGTACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt