Construct: ORF TRCN0000467914
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001859.1_s317c1
- Derived from:
- ccsbBroadEn_02574
- DNA Barcode:
- TAGATACTCTGTCTGCAACCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMAN2 (10960)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467914
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10960 | LMAN2 | lectin, mannose binding 2 | NM_006816.3 | 100% | 100% | |
| 2 | mouse | 66890 | Lman2 | lectin, mannose-binding 2 | NM_025828.3 | 88.1% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggaaggc tggatttggc gttggggctg gggccggcgg tgcctgggaa 121 ggcctgggct tctcggcccc ggccctggcc ccactacacc tctctttctt cttttgttgt 181 tggggtctgt gactgcggat ataactgacg gcaacagtga acatctcaag cgggagcatt 241 cgctcattaa gccctaccaa ggggtcggtt ccagctctat gcccctctgg gacttccagg 301 gcagcactat gctcacgagc cagtacgtac gtctgacccc tgacgagcgc agcaaagagg 361 gctctatctg gaaccaccag ccgtgcttcc tcaaagactg ggaaatgcac gtccacttca 421 aagtccacgg cacagggaag aagaacctcc atggagacgg catcgccttg tggtacaccc 481 gggaccgcct cgtgccaggg cctgtgtttg gaagcaaaga taacttccac ggcttagcca 541 tcttcctgga cacctacccc aatgatgaga ccactgagcg cgtgttcccg tacatctcgg 601 tgatggtgaa caatggctcc ctgtcctacg accacagcaa ggatgggcgc tggaccgagc 661 tggcgggctg cacggctgac ttccgcaacc gcgatcacga caccttcctG GCTGTGCGCT 721 ACTCCCGGGG CCGTCTGACG GTGATGACCG ACCTGGAGGA CAAGAACGAG TGGAAGAACT 781 GCATTGACAT CACGGGAGTG CGCCTGCCCA CCGGCTACTA CTTCGGGGCC TCCGCCGGCA 841 CCGGCGACCT GTCTGACAAT CATGACATCA TCTCCATGAA GCTGTTCCAG CTGATGGTGG 901 AGCACACGCC CGACGAGGAG AGCATCGACT GGACCAAGAT CGAGCCCAGC GTCAACTTCC 961 TCAAGTCGCC CAAAGACAAC GTGGACGACC CCACGGGGAA CTTCCGCAGC GGGCCCCTGA 1021 CGGGGTGGCG GGTGTTCCTG CTGCTGCTGT GCGCTCTCCT GGGCATCGTT GTCTGCGCCG 1081 TGGTGGGGGC CGTGGTGTTC CAGAAGCGGC AGGAGCGGAA CAAGCGCTTC TACTGCCCAA 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAT AGATACTCTG TCTGCAACCT ATCACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att