Construct: ORF TRCN0000467924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009628.1_s317c1
- Derived from:
- ccsbBroadEn_00144
- DNA Barcode:
- GTTTTAATTTCCTTATCGATTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B2M (567)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 567 | B2M | beta-2-microglobulin | NM_004048.3 | 100% | 100% | |
| 2 | human | 567 | B2M | beta-2-microglobulin | XM_005254549.3 | 96.1% | 94.2% | (many diffs) |
| 3 | human | 567 | B2M | beta-2-microglobulin | XR_002957658.1 | 22.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC TCGCTCCGTG GCCTTAGCTG TGCTCGCGCT ACTCTCTCTT TCTGGCCTGG 121 AGGCTATCCA GCGTACTCCA AAGATTCAGG TTTACTCACG TCATCCAGCA GAGAATGGAA 181 AGTCAAATTT CCTGAATTGC TATGTGTCTG GGTTTCATCC ATCCGACATT GAAGTTGACT 241 TACTGAAGAA TGGAGAGAGA ATTGAAAAAG TGGAGCATTC AGACTTGTCT TTCAGCAAGG 301 ACTGGTCTTT CTATCTCTTG TACTACACTG AATTCACCCC CACTGAAAAA GATGAGTATG 361 CCTGCCGTGT GAACCATGTG ACTTTGTCAC AGCCCAAGAT AGTTAAGTGG GATCGAGACA 421 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 541 GGCTTTATAT ATCTTGTGGA AAGGACGAGT TTTAATTTCC TTATCGATTC ATACGCGTTA 601 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt