Construct: ORF TRCN0000467941
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013348.4_s317c1
- Derived from:
- ccsbBroadEn_14837
- DNA Barcode:
- GTGTGCAGTCCAACCCATCTGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL2 (6347)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467941
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6347 | CCL2 | C-C motif chemokine ligand 2 | NM_002982.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 363
- ORF length:
- 297
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agtctctgcc gcccTTCTGT GCCTGCTGCT CATAGCAGCC ACCTTCATTC 121 CCCAAGGGCT CGCTCAGCCA GATGCAATCA ATGCCCCAGT CACCTGCTGT TATAACTTCA 181 CCAATAGGAA GATCTCAGTG CAGAGGCTCG CGAGCTATAG AAGAATCACC AGCAGCAAGT 241 GTCCCAAAGA AGCTGTGATC TTCAAGACCA TTGTGGCCAA GGAGATCTGT GCTGACCCCA 301 AGCAGAAGTG GGTTCAGGAT TCCATGGACC ACCTGGACAA GCAAACCCAA ACTCCGAAGA 361 CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGAGT GTGCAGTCCA ACCCATCTGC GGACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt