Construct: ORF TRCN0000467974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005649.1_s317c1
- Derived from:
- ccsbBroadEn_06825
- DNA Barcode:
- GCTCCACCATCGTGTGTGTCAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PWP2 (5822)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102724159 | LOC102724159 | periodic tryptophan protein... | NM_001368238.1 | 99.9% | 99.8% | 1651T>A |
| 2 | human | 5822 | PWP2 | PWP2 small subunit processo... | NM_005049.3 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 102724159 | LOC102724159 | periodic tryptophan protein... | NR_160695.1 | 83.3% | (many diffs) | |
| 4 | human | 5822 | PWP2 | PWP2 small subunit processo... | XM_011529667.3 | 79.2% | 77% | (many diffs) |
| 5 | mouse | 110816 | Pwp2 | PWP2 periodic tryptophan pr... | NM_029546.2 | 84.2% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2823
- ORF length:
- 2757
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggtatgaa gttcgcttac cggttttcaa atttgctggg tacggtgtac cggcgtggga 121 acctaaattt tacctgcaat ggaaattcag ttatcagtcc cgtgggcaat agagtcactg 181 tatttgacct taaaaacaac aaatctgaca cgctgcccct ggccactcgg tacaacgtca 241 agtgcgtggg gctgtccccg gatggccgcc tcgctatcat cgtcgatgaa gggggcgatg 301 cgctgctggt cagcctggtc tgcaggtctg tgctgcacca cttccacttc aagggctctg 361 tgcacagtgt gtccttctcc cctgatggca ggaagtttgt tgtcacaaag ggtaacattg 421 cccagatgta tcatgcccct gggaagaagc gggagttcaa cgccttcgtt ctggacaaga 481 cctattttgg gccctacgat gagaccacct gcatcgactg gacggatgac tccaggtgct 541 ttgtggttgg gagcaaagac atgtccacct gggtgttcgg agccgagcgc tgggacaacc 601 tcatctacta tgcactgggg ggacataagg atgccatcgt ggcctgcttc tttgaatcca 661 acagcctgga cctgtactca ctcagccagg acggagtgct gtgcatgtgg cagtgtgaca 721 cgccccccga gggcttgcgg ctgaagcccc ctgcgggctg gaaagcagac ctgttgcagc 781 gggaggagga agaggaggag gaggaggacc aggagggcga cagagagacc accatccggg 841 gaaaagccac tccggccgag gaggagaaga caggaaaagt gaagtactca cggctggcca 901 agtacttctt caataaagaa ggggatttta acaacctgac agctgcagca tttcataaga 961 agtctcacct cttggtcact ggctttgctt ctggaatctt ccatcttcat gagctgccag 1021 agtttaacct catccactcc ctgagcatct cagatcagag catcgcctca gtggccatca 1081 atagctcggg ggactggatt gcttttggct gttcaggcct gggccagctg ctggtgtggg 1141 agtggcagag tgagtcctac gtgctcaagc agcagggcca cttcaacagc atggtggccc 1201 tggcctactc gcccgacgga cagtacatcg tgactggcgg ggacgacggc aaggtcaagg 1261 tgtggaacac cctcagcggc ttctgcttcg tcacttttac ggagcactcc agcggggtga 1321 ccggtgtgac ctttactgcc accggctacg ttgtggtgac ctcatccatg gacgggaccg 1381 tgcgagcctt tgaccttcac aggtaccgaa acttccgcac cttcacctct ccacgcccca 1441 cccagttctc ctgtgtggcg gtggatgcga gcggtgagat cgtctctgca ggggcgcagg 1501 actcctttga gattttcgtg tggtccatgc agacaggcag gctccttgat gttttgtctg 1561 gacacgaagg gcccatcagt ggtctgtgtt ttaacccaat gaagtccgtc ctggccagtg 1621 cctcctggga caagacggtg cgcctatggg acatgtttga cagctggagg accaaggaga 1681 cgctggccct gacctctgat gctctggctg tgactattcg ccctgatggt gcggagctgg 1741 ctgtggccac actgaactca cagatcacct tctgggaccc tgagaacgcg gtgcagacgg 1801 gctccattga gggcaggcat gacctcaaga ctggcaggaa ggagctggac aagattacag 1861 ccaagcacgc ggccaagggg aaggccttca ccgccctgtg ctactctgca gacggccaca 1921 gcatcctggc gggaggcatg tccaagttcg tgtgcatcta ccacgtccgt gagcagattc 1981 tcatgaagag gttcgagatc tcttgcaacc tgtctctgga cgccatggag gaatttttga 2041 accgaagaaa aatgacagag tttggcaacc tggcactaat tgatcaggat gctgggcagg 2101 aggatggagt cgcgatacca ctgccaggcg tcaggaaagg tgacatgagt tctcggcact 2161 tcaaacctga gatcagggtg acctcactcc gcttctctcc cactgggcgc tgctgggcgg 2221 ccaccaccac ggagggactc ctcatctact ccctggacac ccgcgtgctc tttgacccgt 2281 ttgagctgga caccagcgtc acccccggga gggtgcgcga ggcactgcgc cagcaggact 2341 tcaccagggc catcctcatg gccctccggc tcaacgagag caaactggtg caggaggccc 2401 tggaggcggt gcccaggggc gagattgaag tggtcaccTC CTCCCTTCCT GAACTGTATG 2461 TGGAGAAAGT GCTGGAGTTT TTAGCTTCCT CCTTTGAAGT GTCTCGCCAC CTGGAATTCT 2521 ACCTCCTCTG GACTCACAAA CTGCTCATGT TGCACGGACA GAAGCTGAAG TCCAGAGCCG 2581 GGACGCTGCT GCCTGTCATT CAGTTCCTCC AGAAGAGCAT CCAGCGGCAC CTGGACGACC 2641 TGTCGAAACT CTGTAGCTGG AACCACTATA ACATGCAGTA CGCACTAGCA GTTTCCAAGC 2701 AGCGGGGCAC AAAACGCTCC CTAGACCCGC TGGGAAGTGA GGAGGAGGCA GAAGCATCTG 2761 AAGATGACAG CCTGCATCTG CTTGGAGGAG GAGGCAGAGA CTCAGAAGAA GAGATGCTGG 2821 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2881 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2941 GGCTTTATAT ATCTTGTGGA AAGGACGAGC TCCACCATCG TGTGTGTCAG CAACGCGTTA 3001 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt