Construct: ORF TRCN0000467997
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008714.1_s317c1
- Derived from:
- ccsbBroadEn_08471
- DNA Barcode:
- GTTTGACGTTACCCGAAACGGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGGF1 (55109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467997
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55109 | AGGF1 | angiogenic factor with G-pa... | NM_018046.5 | 99.8% | 99.5% | 1583C>G;1672A>G;1876A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2208
- ORF length:
- 2142
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcggaggcg ccgtccccgc cgcggtcgcc gccgccgccc acctcccccg 121 agcctgagct ggcccagcta aggcggaagg tggagaagtt ggaacgtgaa ctgcggagct 181 gcaagcggca ggtgcgggag atcgagaagc tgctgcatca cacagaacgg ctgtaccaga 241 acgcagaaag caacaaccag gagctccgca cgcaggtgga agaactcagt aaaatactcc 301 aacgtgggag aaatgaagat aataaaaagt ctgatgtaga agtacaaaca gagaaccatg 361 ctccttggtc aatctcagat tatttttatc agacgtacta caatgacgtt agtcttccaa 421 ataaagtgac tgaactgtca gatcaacaag atcaagctat cgaaacttct attttgaatt 481 ctaaagacca tttacaagta gaaaatgatg cttaccctgg taccgataga acagaaaatg 541 ttaaatatag acaagtggac cattttgcct caaattcaca ggagccagca tctgcattag 601 caacagaaga tacctcctta gaaggctcat cattagctga aagtttgaga gctgcagcag 661 aagcggctgt atcacagact ggatttagtt atgatgaaaa tactggactg tattttgacc 721 acagcactgg tttctattat gattctgaaa atcaactcta ttatgatcct tccactggaa 781 tttattacta ttgtgatgtg gaaagtggtc gttatcagtt tcattctcga gtagatttgc 841 aaccttatcc gacttctagc acaaaacaaa gtaaagataa aaaattgaag aagaaaagaa 901 aagatccaga ttcttctgca acaaatgagg aaaaggattt gaactcagag gatcaaaaag 961 ccttcagtgt tgaacataca agctgcaatg aggaagaaaa tttcgcaaat atgaaaaaga 1021 aggccaaaat aggcattcat cacaaaaata gtccccccaa agtcactgtt ccaactagtg 1081 gaaatactat agagtctcct cttcatgaaa acatctctaa ttcaacatca tttaaagatg 1141 agaaaatcat ggagactgat agtgaaccag aggaaggtga aattacagac tctcagactg 1201 aggatagtta tgacgaagcc attaccagtg aaggcaatgt aactgcagaa gatagtgagg 1261 atgaagatga agacaaaatt tggcccccat gtattagagt aattgtcatt agatcacctg 1321 tgttgcagat aggatcactc tttatcatta ctgctgtaaa ccctgctaca attggaagag 1381 aaaaggatat ggaacatact ctccgaatcc ctgaagttgg tgtcagtaag tttcatgcag 1441 aaatttattt tgaccatgac ttacaaagtt atgtccttgt ggatcaaggc agtcaaaatg 1501 gcacaattgt taatggaaaa cagattcttc agccgaaaac taaatgtgac ccttacgtac 1561 ttgagcatgg agatgaagtc aaaattggag aaactgtctt atcctttcac attcatcctg 1621 gcagtgatac ctgtgatggc tgtgaacgag ggcaggttag agcccacctt cgccttgata 1681 agaaagatga atcttttgtt ggtccaacac taagtaagga ggaaaaagag ttggaaggaa 1741 gaaaagaatt aaagaaaata cgagtaaaat atggtttaca gaatacagaa tacgaagatg 1801 aaaagacatt gaagaatcca aaatataaag atagagctgg aaaacgtagg gagcaggttG 1861 GAAGTGAAGG AACTTTCCAA AGAGATGATG CTCCTGCATC TGTTCATTCT GAAATTACTG 1921 ATAGCAACAA AGGTCGGAAG TTGTTGGAGA AGATGGGTTG GAAGAAAGGA GAGGGCCTGG 1981 GGAAGGATGG TGGAGGAATG AAAACGCCGA TCCAGCTTCA GCTTCGGCGA ACACATGCAG 2041 GCTTGGGGAC AGGCAAACCA TCCTCATTTG AAGATGTTCA CCTTCTCCAA AACAAGAACA 2101 AAAAAAACTG GGACAAAGCA CGAGAGCGGT TTACTGAAAA CTTCCCAGAA ACTAAGCCTC 2161 AAAAAGATGA CCCAGGGACC ATGCCTTGGG TAAAAGGGAC TTTAGAGTGC CCAACTTTCT 2221 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2281 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2341 GTGGAAAGGA CGAGTTTGAC GTTACCCGAA ACGGACGACG CGTTAAGTCg acaatcaacc 2401 tctggattac aaaatttgtg aaagatt