Construct: ORF TRCN0000468022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008684.1_s317c1
- Derived from:
- ccsbBroadEn_10394
- DNA Barcode:
- GCTTTTGTGCCGCGGGAAGTTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KU-MEL-3 (497048)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468022
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 497048 | KU-MEL-3 | uncharacterized LOC497048 | NR_122107.1 | 14.6% | 1_186del;361_1190del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 229
- ORF end:
- 403
- ORF length:
- 174
- Sequence:
-
1 GGGGGGAGGG GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA 61 GGCCAGCTTG GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT 121 GGTTCATTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT 181 GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCACCAT GCTTCCGCGA 241 ACTCCTCGCC CCGACCTCAT CCTTCTCCAG CTCCTACCTG CAGGCCTCAG GCCTCCTGAC 301 CTCCAGGCTC CTTACCCTCC CGCTGGCCTC TGCTCTACAC TCATCTTTGT GCCCTGCTTC 361 CAATCATGCC AGGCCTCGGG GTCCCTGTCT CTAACTTCAC CATTGCCAAC TTTCTTGTAC 421 AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG 481 TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA 541 AAGGACGAGC TTTTGTGCCG CGGGAAGTTA TGACGCGTTA AGTCgacaat caacctctgg 601 attacaaaat ttgtgaaaga tt