Construct: ORF TRCN0000468044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009005.1_s317c1
- Derived from:
- ccsbBroadEn_14990
- DNA Barcode:
- ACCGCCTTAGATGTTGGACATTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IRAK3 (11213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11213 | IRAK3 | interleukin 1 receptor asso... | NM_007199.3 | 99.9% | 99.8% | 439A>G |
| 2 | human | 11213 | IRAK3 | interleukin 1 receptor asso... | NM_001142523.2 | 89.7% | 89.5% | 132_133ins183;256A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1854
- ORF length:
- 1788
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggggaactgt ggggcccgcg gcgcgctgtc ggcgcacacg ctgctgttcg 121 acctgccgcc cgcgctgctc ggagagctct gcgctgttct ggacagctgc gacggcgcgc 181 tgggctggcg cggcctggca gagagacttt caagcagctg gctggatgtt cgtcatattg 241 aaaagtatgt agaccaaggt aaaagtggaa caagagaatt actttggtcc tgggcacaga 301 aaaacaagac catcggtgac cttttacagg tcctccagga gatgggacat cgtcgagcta 361 ttcatttaat tacaaactat ggagcagtgt tgagtccttc agagaagagt tatcaggaag 421 gtggatttcc aaatatatta ttcaaggaaa cagccaatgt caccgtggat aatgttctta 481 ttcctgaaca taatgaaaaa ggagtactgc ttaaatcttc catcagcttt caaaatatca 541 tagaaggaac tagaaatttc cacaaagact tcctaattgg agaaggagag atttttgagg 601 tatacagagt ggagattcaa aacctaacat atgctgtcaa attatttaaa caggagaaaa 661 aaatgcagtg taagaagcat tggaagaggt ttttatctga gcttgaagtt ttactactgt 721 ttcatcaccc aaacatacta gagttggctg catattttac agagactgag aagttctgtc 781 tgatttatcc atacatgaga aatggaacac tttttgacag attgcagtgt gtaggtgaca 841 cggccccact cccttggcac attcgaatcg gtatattaat aggaatatcc aaagccattc 901 actacctgca caacgttcaa ccatgctcgg tcatctgtgg cagtatatca agtgcaaaca 961 tccttttgga tgatcagttt caacccaaac taactgattt tgccatggca cacttccggt 1021 cccacctaga acatcagagt tgtaccataa atatgaccag cagcagcagt aaacatctgt 1081 ggtacatgcc agaagagtac atcagacagg ggaaactttc cattaaaaca gatgtctaca 1141 gctttggaat tgtaataatg gaagttctaa caggatgtag agtagtgtta gatgatccaa 1201 aacatatcca gctgcgggat ctccttagag aattgatgga gaagagaggc ctggattcat 1261 gtctctcatt tctagataag aaagtgcctc cctgccctcg gaatttctct gccaagctct 1321 tctgtttggc aggccggtgt gctgcaacgc gggcaaagtt aagaccatca atggatgaag 1381 ttttaaatac tcttgaaagt actcaagcca gcttgtattt tgctgaagat cctcccacat 1441 cactaaagtc cttcaggtgt ccttctcctc tattcctgga gaatgtacca agtattccag 1501 tggaagatga tgaaagccag aataacaatt tactaccttc tgatgaaggc ctgaggatag 1561 acagaatgac tcagaaaact ccttttGAAT GCAGCCAGTC TGAGGTTATG TTTCTGAGCT 1621 TGGACAAAAA GCCAGAGAGC AAGAGAAATG AGGAAGCTTG CAACATGCCC AGTTCTTCTT 1681 GTGAAGAAAG TTGGTTCCCA AAGTATATAG TTCCATCCCA GGACTTAAGG CCCTATAAGG 1741 TAAATATAGA TCCTTCTTCA GAAGCTCCAG GGCATTCTTG CAGGAGCAGG CCAGTGGAGA 1801 GCAGCTGTTC CTCCAAATTT TCCTGGGATG AATATGAACA GTACAAAAAA GAATACCCAA 1861 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1921 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1981 TATCTTGTGG AAAGGACGAA CCGCCTTAGA TGTTGGACAT TCGACGCGTT AAGTCgacaa 2041 tcaacctctg gattacaaaa tttgtgaaag att