Construct: ORF TRCN0000468067
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002685.1_s317c1
- Derived from:
- ccsbBroadEn_02675
- DNA Barcode:
- CATGAGGCACTGATGGACGCTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CTRC (11330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468067
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11330 | CTRC | chymotrypsin C | NM_007272.3 | 100% | 100% | |
| 2 | human | 11330 | CTRC | chymotrypsin C | XM_011540550.1 | 66.7% | 56.3% | 493_494ins146;659_840del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 870
- ORF length:
- 804
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gggcatcact gtcctcgctg cgctcttggc ctgtgcctcc agctgtgggg 121 tgcccagctt cccgcccaac ctatccgccc gagtggtggg aggagaggat gcccggcccc 181 acagctggcc ctggcagatc tccctccagt acctcaagaa cgacacgtgg aggcatacgt 241 gtggcgggac tttgattgct agcaacttcg tcctcactgc cgcccactgc atcagcaaca 301 cccggaccta ccgtgtggcc gtgggaaaga acaacctgga ggtggaagac gaagaaggat 361 ccctgtttgt gggtgtggac accatccacg tccacaagag atggaatgcc ctcctgttgc 421 gcaatgatat tgccctcatc aagcttgcag agcatgtgga gctgagtgac accatccagg 481 tggcctgcct gccagagaag gactccctgc tccccaagga ctaccccTGC TATGTCACCG 541 GCTGGGGCCG CCTCTGGACC AACGGCCCCA TTGCTGATAA GCTGCAGCAG GGCCTGCAGC 601 CCGTGGTGGA TCACGCCACG TGCTCCAGGA TTGACTGGTG GGGCTTCAGG GTGAAGAAAA 661 CCATGGTGTG CGCTGGGGGC GATGGCGTCA TCTCAGCCTG CAATGGGGAC TCCGGTGGCC 721 CACTGAACTG CCAGTTGGAG AACGGTTCCT GGGAGGTGTT TGGCATCGTC AGCTTTGGCT 781 CCCGGCGGGG CTGCAACACC CGCAAGAAGC CGGTAGTCTA CACCCGGGTG TCCGCCTACA 841 TCGACTGGAT CAACGAGAAA ATGCAGCTGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 901 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 961 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACATGA 1021 GGCACTGATG GACGCTGTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1081 tgaaagatt