Construct: ORF TRCN0000468145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007209.1_s317c1
- Derived from:
- ccsbBroadEn_12442
- DNA Barcode:
- AAGCAATGACCAGACATTCTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OXCT2 (64064)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468145
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64064 | OXCT2 | 3-oxoacid CoA-transferase 2 | NM_022120.2 | 50% | 48.3% | (many diffs) |
| 2 | human | 192217 | OXCT2P1 | 3-oxoacid CoA-transferase 2... | NR_132962.1 | 43% | 1_90del;868_1804del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgctgcgg ctcctggcgt cagtgctcgg gcgcggggtc cccgccggcg 121 gctcagggct cgcgctgtcc cagggctgcg cccgctgctt tgccaccagt ccccggcccc 181 gtgccaagtt ctacgcggac ccggtggaga tggtgaagga catctctgac ggggcgaccg 241 tcatgatcgg gggcttcggg ctctgcggga tccccgagaa cctgatcgcc gcgctgctca 301 ggacccgcgt gaaagacctg caggtggtca gcagcaacgt gggcgtggag gacttcggcc 361 tgggcctcct gctggccgcc aggcaggtcc gtcgcatcgt ctgttcctac gtgggcgaga 421 acaccctgtg cgagagccag tacctggcag gagagctgga gctggagctc acgccccagg 481 gcaccctggc cgagcgcatc cgcgcggggg gcgccggggt gcccgccttc tacacCCCCA 541 CGGGCTACGG GACCCTGGTC CAGGAAGGGG GCGCCCCCAT CCGCTACACC CCGGACGGCC 601 ACCTGGCGCT CATGAGCCAG CCCCGAGAGG TGAGGGAGTT CAACGGCGAC CACTTCCTTT 661 TGGAGCGCGC CATCCGGGCA GACTTCGCCC TGGTGAAAGG GTGGAAGGCC GACCGGGCAG 721 GAAACGTGGT CTTCAGGAGA AGCGCCCGCA ATTTCAACGT GCCCATGTGC AAAGCTGCAG 781 ACGTCACGGC GGTGGAGGTG GGGGCTTCCC CCCAGAAGAC ATCCACGTTC CTAACATTTA 841 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGAAA GCAATGACCA GACATTCTTC ATACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt