Construct: ORF TRCN0000468160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008364.1_s317c1
- Derived from:
- ccsbBroadEn_12650
- DNA Barcode:
- CATTTTCAGTGACCTTGCGCCGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCD5 (79966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79966 | SCD5 | stearoyl-CoA desaturase 5 | NM_024906.2 | 50.7% | 45.3% | (many diffs) |
| 2 | human | 79966 | SCD5 | stearoyl-CoA desaturase 5 | NM_001037582.3 | 39.3% | 35.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aggcccggcc accgacgcgg ggaagatccc ttTCTGCGAC GCCAAGGAAG 121 AAATCCGTGC CGGGCTCGAA AGCTCTGAGG GCGGCGGCGG CCCGGAGAGG CCAGGCGCGC 181 GCGGGCAGCG GCAGAACATC GTCTGGAGGA ATGTCGTCCT GATGAGCTTG CTCCACTTGG 241 GGGCCGTGTA CTCCCTGGTG CTCATCCCCA AAGCCAAGCC ACTCACTCTG CTCTGGGCCT 301 ACTTCTGCTT CCTCCTGGCC GCTCTGGGTG TGACAGCTGG TGCCCATCGC TTGTGGAGCC 361 ACAGGTCCTA CCGGGCCAAG CTGCCTCTGA GGATATTTCT GGCTGTCGCC AACTCCATGG 421 CTTTCCAGGC AGCAGCAGAA GTGGTCCTGA CCCAGATGCC CTGCCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGACAT TTTCAGTGAC CTTGCGCCGC TACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t