Construct: ORF TRCN0000468293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001904.1_s317c1
- Derived from:
- ccsbBroadEn_02170
- DNA Barcode:
- GGTCTGACACACTTCCGGAGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKAP7 (9465)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468293
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9465 | AKAP7 | A-kinase anchoring protein 7 | NM_004842.4 | 100% | 100% | |
| 2 | human | 9465 | AKAP7 | A-kinase anchoring protein 7 | NM_138633.3 | 77.8% | 77.8% | 50_118del |
| 3 | human | 9465 | AKAP7 | A-kinase anchoring protein 7 | NM_016377.4 | 20.8% | 19% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 309
- ORF length:
- 243
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGG CCAGCTTTGC TGCTTTCCTT TCTCAAGAGA TGAAGGAAAA ATCAGTGAAA 121 AGAACGGAGG GGAGCCCGAT GACGCTGAAC TAGTAAGGCT CAGTAAGAGG CTGGTGGAGA 181 ACGCGGTGCT CAAGGCTGTC CAGCAGTATC TGGAGGAAAC ACAGAATAAA AACAAGCCGG 241 GGGAGGGGAG CTCTGTGAAA ACCGAAGCAG CTGATCAGAA TGGCAATGAC AATGAGAACA 301 ACAGGAAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 361 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 421 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGTCTG ACACACTTCC GGAGGACAAC 481 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt