Construct: ORF TRCN0000468327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007848.1_s317c1
- Derived from:
- ccsbBroadEn_13467
- DNA Barcode:
- ATAATACACCTAGCCTCTTATCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP46 (54777)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54777 | CFAP46 | cilia and flagella associat... | NM_001200049.3 | 8.8% | 8.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cctggtcatc acgcaggagc tggcccgcgc cgagagccag caagatgctg 121 cgtccttgaa gaaggcctac gagttgatca aatcggccaa cctagggaaa tcggagtttg 181 acccctcaga gagcttcagc ccagacctgt ttgttctgtg tgcagagcag gccctgaaga 241 tgaggcagcc agaggtgagc gaggactgca tccaaatgta cttcaaggtg aaggcgccca 301 tcacccagtt tctgggccga gcgcacctgt gcagggccca gatgtgtgcc ccGAAGTCGG 361 CAGAAAACCT GGAGGAATTT GAAAATTGCG TGACTGAGTA CATGAAGGCC ATAAACTTTG 421 CCAAAGGAGA ACCGAGGTAC TACTTTTTGG TGTACAATGC ATCAGTCCTC TACTGGCAGA 481 TGGTGAGGCC GTTCCTCAAG CCTGGATATC GTCACCATCT GATCCCCAGC CTTTCCCAAA 541 TCATAAACGT GCTGAGTCAG ACTGAGGAGG AAGACAAGGA GTGGCGTGCT GAGCTGATGC 601 TGGAACTTCT GGAGTGTTAT CTGCAAGCCG GAAGAAAGGA GGAGGCTGCC AGGTTCTGCT 661 CCACGGCAGC TCCGTTCATT AAGTCTCACG TGCCACAGAA ATACCGGCAG ATATTCTCTG 721 TTATGGTAAA TCAGCGTATT AGAAATAGAG ACGTAAGAAG TAATTTTCTT AAAAGTCATT 781 GTTCAGAAAA CTGCGTAGAA ATTACATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATAATACA 961 CCTAGCCTCT TATCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt