Construct: ORF TRCN0000468374
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007680.1_s317c1
- Derived from:
- ccsbBroadEn_04585
- DNA Barcode:
- TAAGCCTATCTGAGGCAGCTCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTD1 (92675)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468374
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92675 | DTD1 | D-aminoacyl-tRNA deacylase 1 | NM_080820.6 | 100% | 100% | |
| 2 | human | 92675 | DTD1 | D-aminoacyl-tRNA deacylase 1 | XM_005260872.4 | 78.9% | 78.9% | 0_1ins132 |
| 3 | human | 92675 | DTD1 | D-aminoacyl-tRNA deacylase 1 | NM_001318043.2 | 78.6% | 68.3% | (many diffs) |
| 4 | mouse | 66044 | Dtd1 | D-tyrosyl-tRNA deacylase 1 | NM_025314.3 | 88.3% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 696
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggccgtg gtgcagcgcg tcacccgggc cagcgtcaca gttggaggag 121 agcagattag tgccattgga aggggcatat gtgtgttgct gggtatttcc ctggaggata 181 cgcagaagga actggaacac atggtccgaa agattctaaa ccTGCGTGTA TTTGAGGATG 241 AGAGTGGGAA GCACTGGTCG AAGAGTGTGA TGGACAAACA GTACGAGATT CTGTGTGTCA 301 GCCAGTTTAC CCTCCAGTGT GTCCTGAAGG GAAACAAGCC TGATTTCCAC CTAGCAATGC 361 CCACGGAGCA GGCAGAGGGC TTCTACAACA GCTTCCTGGA GCAGCTGCGT AAAACATACA 421 GGCCGGAGCT TATCAAAGAT GGCAAGTTTG GGGCCTACAT GCAGGTGCAC ATTCAGAATG 481 ATGGGCCTGT GACCATAGAG CTGGAATCGC CAGCTCCCGG CACTGCTACC TCTGACCCAA 541 AGCAGCTGTC AAAGCTCGAA AAACAGCAGC AGAGGAAAGA AAAGACCAGA GCTAAGGGAC 601 CTTCTGAATC AAGCAAGGAA AGAAACACTC CCCGAAAAGA AGACCGCAGT GCCAGCAGCG 661 GGGCTGAGGG CGACGTGTCC TCTGAACGGG AGCCGTTGCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 ATAAGCCTAT CTGAGGCAGC TCTTGACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt