Construct: ORF TRCN0000468378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012425.1_s317c1
- Derived from:
- ccsbBroadEn_06765
- DNA Barcode:
- TAATGGGGAATAACCTTGGACAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRCC (5546)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468378
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5546 | PRCC | proline rich mitotic checkp... | NM_005973.5 | 99.8% | 99.5% | 7C>G;1135C>A |
| 2 | human | 5546 | PRCC | proline rich mitotic checkp... | XM_005245313.3 | 93.7% | 93.4% | 7C>G;1084_1179del;1231C>A |
| 3 | human | 5546 | PRCC | proline rich mitotic checkp... | XM_005245314.2 | 93.4% | 93.2% | 7C>G;1083_1084ins96 |
| 4 | human | 5546 | PRCC | proline rich mitotic checkp... | XM_011509726.2 | 80.2% | 74.5% | (many diffs) |
| 5 | human | 5546 | PRCC | proline rich mitotic checkp... | XR_921868.3 | 68.8% | (many diffs) | |
| 6 | mouse | 94315 | Prcc | papillary renal cell carcin... | NM_033573.2 | 88.7% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1539
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggtggttgct tacgccagca gcgatgagag cgagccggat gaggctgagc 121 ccgagccgga ggaagaggag gcggtggctc ctacatctgg gcccgcttta gggggcttgt 181 tcgcttctct ccctgcgccc aagggtccgg ccttgctgcc tccgccccct cagatgctgg 241 cgccagcctt tcccccgccg ctgttgcttc ccccacccac cggagacccc aggcttcagc 301 ctcctccccc cttgcccttc ggcctgggag gcttcccccc acctccaggc gtgagcccgg 361 ctgaagcggc gggagttggg gagggactgg gattggggtt gccctcgccc cgaggccctg 421 gcctcaatct gccccctcca attggcggtg ccggtccccc gctggggctt cccaagccaa 481 agaagaggaa agagcccgtg aagatcgcgg cgccggagtt gcataaggga gattcagatt 541 ctgaggaaga tgaacccaca aagaagaaaa ctatccttca gggatccagt gaggggactg 601 gtttgtctgc cttgcttccc caacctaaaa acctgactgt gaaagagact aacaggttgc 661 tcctgcccca tgccttctcc cgcaaaccct cggatggctc ccctgatact aagccctcca 721 gactggcttc taagaccaag acttcctctc ttgcccctgt tgtgggcacc acaaccacca 781 ctccgtcgcc ctctgctatc aaggctgctg ccaagagtgc tgccctgcag gtgacaaagc 841 agatcacgca ggaagaagac gacagtgatg aggaagtagc ccccgaaaac tttttctccc 901 tccctgaaaa ggctgagcca cctggagttg agccataccc ttaccccatc cccactgtcc 961 ctgaagagct gcctccaggc acggaaccag agccggcttt ccaggacgat gcagccaatg 1021 ccccccttga attcaagatg gcagcaggtt caagtggggc cccttggatg cctaagcctg 1081 gggacgacta cagctacaat cagttttcca catatGGCGA TGCCAATGCC GCTGGTGCTT 1141 ATTATCAGGA TTATTACAGT GGTGGCTACT ATCCTGCACA GGACCCGGCC CTGGTCCCCA 1201 CCCAGGAAAT TGCCCCAGAT GCCTCCTTCA TCGATGACGA AGCATTTAAG CGGCTGCAGG 1261 GCAAGAGGAA CCGAGGGAGA GAAGAAATCA ACTTTGTGGA GATCAAAGGT GATGACCAGC 1321 TCAGTGGGGC CCAGCAATGG ATGACTAAGT CATTGACAGA AGAGAAAACC ATGAAGTCAT 1381 TCAGCAAAAA GAAAGGTGAG CAGCCAACAG GCCAGCAGCG GCGGAAACAC CAGATCACAT 1441 ATCTTATTCA TCAGGCCAAG GAGCGGGAGC TGGAACTGAA GAACACCTGG TCAGAGAACA 1501 AGCTCAGCCG CCGTCAGACC CAAGCCAAAT ATGGATTCTA CCCCACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGATAATGG GGAATAACCT TGGACAGGAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt