Construct: ORF TRCN0000468446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015261.1_s317c1
- Derived from:
- ccsbBroadEn_06384
- DNA Barcode:
- CTGTTGTTGGAGAGCTGCTCGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMOX1 (3162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3162 | HMOX1 | heme oxygenase 1 | NM_002133.3 | 99.8% | 99.6% | 338G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcgtccgcaa cccgacagca tgccccagga tttgtcagag gccctgaagg 121 aggccaccaa ggaggtgcac acccaggcag agaatgctga gttcatgagg aactttcaga 181 agggccaggt gacccgagac ggcttcaagc tggtgatggc ctccctgtac cacatctatg 241 tggccctgga ggaggagatt gagcgcaaca aggagagccc agtcttcgcc cctgtctact 301 tcccagaaga gctgcaccgc aaggctgccc tggagcagga cctggccttc tggtacgggc 361 cccgctggca ggaggtcatc ccctacacac cagccatgca gcactatgtg aagcggctcc 421 acgaggtggg gcgcacagag cccgagctgc tggtggccca cgcctacacc cgctacctgg 481 gtgacctgtc tgggggccag gtgctcaaaa agattgccca gaaagccctg gacctgccca 541 gctctggcga gggcctggcc ttcttcacct tccccaacat tgccagtgcc accaagttca 601 agcagctcta ccgctcccgc atgaactccc tggagatgac tcccgcagtc aggcagaggg 661 tgatagaaga ggccaagact gcgttcctgc tcaacatcca gctctttgag gagttgcagg 721 agctgctgac ccatgacacc aaggaccaga gcccctcacg ggcaccaggg cttcgccagc 781 gggccagcaa caaagtgcaa gattctgccc ccgtggagac tcccagaggg aagcccccac 841 tcaacacccg ctcccaggct ccgcttctcc gatgggtcct tacactcagc tttcTGGTGG 901 CGACAGTTGC TGTAGGGCTT TATGCCATGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTGTT 1081 GTTGGAGAGC TGCTCGAATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt