Construct: ORF TRCN0000468506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008729.1_s317c1
- Derived from:
- ccsbBroadEn_01685
- DNA Barcode:
- ATGTATTACCTCTTTGCCGGTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFAIP1 (7126)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7126 | TNFAIP1 | TNF alpha induced protein 1 | NM_021137.5 | 100% | 100% | |
| 2 | human | 7126 | TNFAIP1 | TNF alpha induced protein 1 | XM_017024993.2 | 80.8% | 62% | 714_835del;1071_1173del |
| 3 | mouse | 21927 | Tnfaip1 | tumor necrosis factor, alph... | NM_001159392.1 | 91.3% | 97.1% | (many diffs) |
| 4 | mouse | 21927 | Tnfaip1 | tumor necrosis factor, alph... | NM_009395.4 | 91.3% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gggggacacc tgcctgtgcc cagcctcagg ggccaagccc aagctcagtg 121 gcttcaaggg aggagggttg ggcaacaagt atgtccagct caacgtgggc ggctctctgt 181 actacaccac tgtgcgggcc ctgacccgcc acgacaccat gctcaaggcc atgttcagtg 241 ggcgcatgga ggtgctgacc gacaaagaag gctggatcct catagaccgt tgtggaaagc 301 actttggcac cattttgaat tacctccgag atgacaccat caccctccct cagaaccggc 361 aagaaatcaa ggaattgatg gctgaagcaa agtattacct catccagggg ctggtgaata 421 tgtgccagag tgccctgcag gacaagaagg actcctacca gcctgtgtgc aacatcccca 481 tcatcacatc cctaaaggag gaggagcggc tcatcgaatc ctccaccaag cccgtggtga 541 agctgctgta caacagaagc aacaacaagt attcctacac cagcaactct gacgaccacc 601 tgctgaaaaa catcgagctg tttgacaagc tctccctgcg cttcaacggc cgcgtgctct 661 tcatcaagga tgtcattggt gacgagatct gctgctggtc ctttTATGGC CAGGGCCGTA 721 AGCTGGCAGA GGTGTGCTGT ACCTCCATCG TGTATGCCAC GGAGAAGAAG CAGACCAAGG 781 TGGAATTCCC AGAGGCCCGA ATCTATGAGG AGACACTCAA CGTCCTACTC TATGAGACTC 841 CCCGCGTCCC CGACAACTCC TTGTTGGAGG CCACAAGCCG TAGCCGCAGC CAGGCTTCCC 901 CCAGTGAAGA TGAGGAGACC TTTGAACTGC GGGACCGTGT CCGCCGCATC CACGTCAAGC 961 GCTACAGCAC TTACGATGAC CGGCAGCTCG GCCACCAGTC TACCCATCGC GACTGCCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAA TGTATTACCT CTTTGCCGGT CGCACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att