Construct: ORF TRCN0000468518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002687.1_s317c1
- Derived from:
- ccsbBroadEn_01509
- DNA Barcode:
- GTTCCCTCAGCCTTTAACTGCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XCL1 (6375)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468518
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6375 | XCL1 | X-C motif chemokine ligand 1 | NM_002995.3 | 100% | 100% | |
| 2 | human | 6375 | XCL1 | X-C motif chemokine ligand 1 | XM_011509865.2 | 100% | 100% | |
| 3 | human | 6846 | XCL2 | X-C motif chemokine ligand 2 | NM_003175.4 | 98.5% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg taCAAAAAAG 61 TTGGCATGAG ACTTCTCATC CTGGCCCTCC TTGGCATCTG CTCTCTCACT GCATACATTG 121 TGGAAGGTGT AGGGAGTGAA GTCTCAGATA AGAGGACCTG TGTGAGCCTC ACTACCCAGC 181 GACTGCCGGT TAGCAGAATC AAGACCTACA CCATCACGGA AGGCTCCTTG AGAGCAGTAA 241 TTTTTATTAC CAAACGTGGC CTAAAAGTCT GTGCTGATCC ACAAGCCACA TGGGTGAGAG 301 ACGTGGTCAG GAGCATGGAC AGGAAATCCA ACACCAGAAA TAACATGATC CAGACCAAGC 361 CAACAGGAAC CCAGCAATCG ACCAATACAG CTGTGACTCT GACTGGCTAC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGAGTTCCCT CAGCCTTTAA CTGCTCCACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt